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Model Organism Database funding is in trouble: Let your voice be heard

Posted by on June 23rd, 2016

  Model Organisms such as yeast, worm, fly, fish, rat, and mouse are key drivers of biological research, providing manipulable and cost-effective experimental systems that continuously yield fundamental insights into human biology and health. These discoveries rely on the accumulated wealth of genetic, genomic and cellular knowledge for each organism, which is made accessible via[…]

Sweetening with a pinch of salt: maximized Cas9 efficiency in zebrafish

Posted by on June 14th, 2016

  Alexa Burger, Mosimann lab, Institute of Molecular Life Sciences, University of Zürich, Switzerland. When I first heard about the “new” genome editing method in early 2013 called CRISPR-Cas9, I thought: “Never ever again will I work with targeted nucleases!” Now it’s mid-2016, we published our approaches to maximize Cas9 effectiveness in zebrafish with Development[…]

Improving the design of animal experiments: Introducing the Experimental Design Assistant (EDA)

Posted by on June 7th, 2016

This post was originally published as a Newsletter article from ShARM (Shared Ageing Research Models) Scientists using animals in research have a responsibility to ensure that the studies are appropriately designed, conducted, analysed and reported so that they impartially and robustly answer the question they are intended to, and truly add to the knowledge base. Unfortunately there[…]

Geometrical models of tissue as tools for uncovering rules underlying tissue organization

Posted by on May 27th, 2016

A major challenge in cell and developmental biology is to understand the mechanisms whereby cells interact with each other to form the variety of complex tissue forms present in organisms. This requires visualizing and analysing different cellular processes across multiple scale levels -from the subcellular to the tissue, i.e. generating cell and tissue models at[…]

Scratching the surface of a rainbow

Posted by on April 26th, 2016

  Why some vertebrates like salamanders and zebrafish are able to regenerate complex tissues while humans cannot is a question that has fascinated biologists for centuries. Understanding how and why regeneration occurs in these animals can inspire novel treatment strategies for regenerative medicine. At the cellular level, the regeneration process is driven by dynamic activities[…]

Light sheet microscopy 101: Get started with a short video protocol

Posted by on April 13th, 2016

Here you can find out more about our video protocol on using light sheet microscopy to image zebrafish eye development.   Light sheet fluorescence microscopy has quickly become a popular technique in developmental biology. This method is very gentle to the samples, with fast acquisition speed and allows capturing the samples from any angle or from[…]

Labome releases Validated Antibody Database (VAD) version 2.2

Posted by on March 22nd, 2016

Developmental biologists use antibodies extensively to study the gene expression during different stages.  However, there is a lack of specific antibodies against many proteins related to development.  In addition, some antibodies yield unspecific and/or irreproducible results.   To help alleviate this antibody quality and specificity problem, Labome sought to organize antibody applications cited in formal[…]

Developing the auxin-inducible degradation (AID) system for versatile conditional protein depletion in C. elegans

Posted by on March 19th, 2016

By Liangyu Zhang and Abby F. Dernburg    The nematode Caenorhabidis elegans is among the most widely used and powerful model organisms for studying mechanisms underlying cellular and developmental processes. Although a variety of approaches for conditional protein expression have been developed in C. elegans, available tools for conditional protein depletion are far more limited,[…]

drosophila.me – manage your fly stocks and crosses

Posted by on February 26th, 2016

Some time ago I wrote about a webpage I’ve created to manage genetic fly crosses. In that past post, I’ve promised a new version of the software, and I’m happy to announce it here. The software can be reached under the address http://drosophila.me. Many many things changed from the previous version, some features: User accounts[…]

Moving proteins within living embryos using light

Posted by on February 24th, 2016

By Clare Buckley and Rachel Moore One of the things that we find most challenging about working with whole vertebrate organisms is how we can tie ourselves in knots trying to unpick the function of a single component within such an intricate and interconnected web of proteins and signalling cascades. All too often knocking out[…]