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Scratching the surface of a rainbow

Posted by on April 26th, 2016

  Why some vertebrates like salamanders and zebrafish are able to regenerate complex tissues while humans cannot is a question that has fascinated biologists for centuries. Understanding how and why regeneration occurs in these animals can inspire novel treatment strategies for regenerative medicine. At the cellular level, the regeneration process is driven by dynamic activities[…]

Light sheet microscopy 101: Get started with a short video protocol

Posted by on April 13th, 2016

Here you can find out more about our video protocol on using light sheet microscopy to image zebrafish eye development.   Light sheet fluorescence microscopy has quickly become a popular technique in developmental biology. This method is very gentle to the samples, with fast acquisition speed and allows capturing the samples from any angle or from[…]

Labome releases Validated Antibody Database (VAD) version 2.2

Posted by on March 22nd, 2016

Developmental biologists use antibodies extensively to study the gene expression during different stages.  However, there is a lack of specific antibodies against many proteins related to development.  In addition, some antibodies yield unspecific and/or irreproducible results.   To help alleviate this antibody quality and specificity problem, Labome sought to organize antibody applications cited in formal[…]

Developing the auxin-inducible degradation (AID) system for versatile conditional protein depletion in C. elegans

Posted by on March 19th, 2016

By Liangyu Zhang and Abby F. Dernburg    The nematode Caenorhabidis elegans is among the most widely used and powerful model organisms for studying mechanisms underlying cellular and developmental processes. Although a variety of approaches for conditional protein expression have been developed in C. elegans, available tools for conditional protein depletion are far more limited,[…]

drosophila.me – manage your fly stocks and crosses

Posted by on February 26th, 2016

Some time ago I wrote about a webpage I’ve created to manage genetic fly crosses. In that past post, I’ve promised a new version of the software, and I’m happy to announce it here. The software can be reached under the address http://drosophila.me. Many many things changed from the previous version, some features: User accounts[…]

Moving proteins within living embryos using light

Posted by on February 24th, 2016

By Clare Buckley and Rachel Moore One of the things that we find most challenging about working with whole vertebrate organisms is how we can tie ourselves in knots trying to unpick the function of a single component within such an intricate and interconnected web of proteins and signalling cascades. All too often knocking out[…]

FISHing fish

Posted by on February 15th, 2016

By L. Carine Stapel and Nadine L.Vastenhouw   In developmental biology, the ability to analyze gene expression patterns is essential to address questions about gene regulation and cell fate. In our lab, we use zebrafish embryos to ask questions such as how is the onset of transcription in an embryo temporally regulated? How do cells[…]

New 3D anatomy viewer available at eMouseAtlas

Posted by on January 26th, 2016

A new 3D viewer that allows interactive visualisation of mouse embryo anatomy is now available from the eMouseAtlas website (www.emouseatlas.org/). A slice viewer allows visualisation of anatomy on arbitrary section through mouse embryos – much like a virtual microtome – whilst a 3D anatomy pop-up window allows users to visualise the delineated anatomical components in[…]

Rapid electron microscopic detection of GFP-tagged proteins in cells and whole organisms

Posted by on December 23rd, 2015

The use of green fluorescent protein (GFP) has revolutionised the study of dynamic cellular processes in cells, tissues, and whole organisms. Laboratories throughout the world have exploited the simplicity of GFP as an everyday tool to determine protein localisation in cell lines and whole organisms. Fluorescence microscopy now dominates the imaging field although the attainable[…]

Computing the worm: artificial intelligence approaches to planarian regeneration and beyond

Posted by on October 30th, 2015

Pattern formation and regulation emerges from cellular activity determined by specific biophysical and genetic rules. A major challenge for developmental biology, biomedicine, and synthetic bioengineering is this highly indirect (Lobo et al., 2014b) relationship between the rules that govern processes at the lower scales, and the anatomical outcomes observed at the macroscopic scale. It is[…]