#stemcellfacts – Tweeting stem cell research

Posted by on February 16th, 2012

Twitter logoAt the start of February, EuroStemCell used our Twitter page @eurostemcell in a new way: We posted a series of fascinating facts and ‘test your knowledge’ questions about stem cells, using the hashtag #stemcellfacts. The tweets covered a lot of ground, from embryonic stem cells and blastocysts to skin stem cells, gut stem cells, heart cells and regeneration.

Thanks to Kate Blair for developing the #stemcellfacts concept and researching the content for the 30 tweets. You can see all the tweets collated with responses from other tweeters in our Storify summary.

We’ve got off to a flying start in 2012 with lots of other activities too - new blogs, translation into Italian, new educational tools and articles about embryonic stem cells. Find out more in our February newsletter. And as ever, we’re keen to hear you feedback at www.eurostemcell.org/contact.
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Bio Web Conferences – Critical discussions with developmental biologists for deep learning.

Posted by on February 10th, 2012

Dear Developmental biology community,

I would like to bring to your attention a potentially valuable resource for your teaching and research endeavors.  I am a neurodevelopmental biologist at Smith College.  I started teaching a course in Developmental Biology back in 2005, and since then have been utilizing web conferencing technology to bring the research behind concepts alive in the classroom.  My students have been interacting with leading scientists in the field of developmental biology holding organized Q&A video conferences focused on current and seminal research articles.  I am posting this to the Node as since I started using this pedagogical approach I have been recording these discussions, and with full consent provided, I have established an online repository of these recordings via my lab website.  I have each conference (40 now and growing) organized by topic for ease of searching, and each individual session is further broken down by specific question to facilitate quick access to your greatest interest.

Because these sessions are based on key research papers they are extremely applicable for any teacher or student to use in their own courses as supplemental resources to what is probably the very same topics being covered.  For instance, I often assign my students select conferences to watch to supplement their readings or coverage of the material.  Moreover, in class I will poise certain questions about a topic to my student and after some discussion, click on say, Dr. Cliff Tabin’s response to the similar question.  It provides a new and real perspective to the information that students truly appreciate and fosters long-term retention of the material.

There are also many other positive outcomes to both conducting and watching these conferences.  Namely students gain a very different and revealing perspective of not only where a particular field of Dev Bio is moving, but more personal understandings of who the scientists are and how they got to where they are today.  Listening to these remarkable scientists articulate their thinking process to address the research question is extremely illuminating to the developing scientist in your classroom.

So I invite and encourage you to check out these discussions as I am disseminating them for your benefit and use.  I hope you find them helpful.  Feel free to let me know what you think and, if you like them, how you might use them in your teaching.

“Bio Web Conferences” http://sophia.smith.edu/~mbarresi/lab/biowebconferences.html

Best regards,

Michael J.F. Barresi

P.S. additional post on stem cell documentaries coming….

 
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Freeware for scientists

Posted by on February 6th, 2012

After commenting on a previous post, I’ve decided to make my own post about freeware I use regularly that other scientists might find useful. All of these are available for Windows, Mac and Linux as far as I’m aware and come with various price plans if you want more storage space/functionality. You’ll need to create an account for some of them, but again that’s free.

Libre Office – I use my own laptop in the lab and as a result of that I don’t have some common software like Photoshop, Illustrator and most annoyingly, Microsoft Office. Rather than paying out of my nose for a productivity suite like Office, I chose to have a go at some open source alternatives. LibreOffice is an open source productivity suite that’s spent around 20 years in development, so it’s pretty stable. It comes with the same kind of programs as Office, a word processor, spreadsheets, presentation maker, drawing tools etc. It’s also compatible with MO as well, so you can open documents you created in program with the other. Migrating from Microsoft to LibreOffice was pretty easy and most of the layout and functionality is the same. I’ve been using this for a year or so now and I think whilst it still lacks the polish of Microsoft Office, it works just as well.

AlternativesGoogle Docs, laTEX (this is supposed to be specific for creating manuscripts)

Dropbox – I mentioned this in a comment on a previous post, but I’ll go into a little more detail here. Dropbox is basically a file syncronisation tool that you can download as a client program. Once installed, if you place a file in your Dropbox folder, it syncs it with your account so you can access that file from anywhere with an internet connection. It’s very easy to use and once you set up your Dropbox folder, you can share it with anyone. The syncronisation between folders on different computers is very fast, you can upload a file onto a shared Dropbox folder and within seconds the other person receives the file. This is very useful for collaborations or even just sharing data in a lab.

The servers they use to store files are very secure and you can create multiple sub-folders that you can share with various people. You get 2GB free storage but if you invite other people to share your folders or install it on another computer you get some extra storage.

AlternativesSugarsync

Mendeley – Mendeley is a web based reference manager that also has a desktop app you can download to organise PDFs and documents on your computer. The web app lets you build a library with an easy to use web importer that works as a plug-in to your browser. Mendeley also store pdfs on the (up to 500MB) and you can retrieve them from any computer or share them with other users by forming groups.

The Mendeley desktop app organises and indexed your PDFs that are stored on your computer. You can also annotate, highlight and add sticky notes to your files. As I begin to write my thesis, I’m finding this part increasingly useful. There is also a toolbar you can install in Microsoft Word or LibreOffice to cite papers whilst writing.

Unlike Endote, it’s free (although you can pay for more cloud storage) and unlike Papers, it’s a cross-platform tool (available for Windows, Mac and Linux), making it very useful for collaborations. I find the interface really easy to use and within half-hour of downloading it, I had most of my references stored on the desktop app. Also the ability to annotate and make notes on papers is proving to be invaluable.

Alternatives - Zotero

Reflect – This is a useful look-up tool when reading papers online. Basically, it’s a plugin for your browser that highlights proteins/molecules/biological concepts in any text. You can click on the highlighted text to show a pop-up window which displays some basic information such as what the molecule is, it’s role, structure and what it interacts with. The information displayed is community driven so for really obscure proteins and molecules there might not be a lot of information displayed, but the makers are keen on the community using it to edit and add more information.

Doodle – Doodle is a web-based time management tool that you can use to co-ordinate meetings. It does this by creating simple polls where everyone can vote on when they are free. You can use various calender programs like iCal, Microsoft Outlook or Google Calender to track dates and organise meetings with other people. I’ve never used it professionally, only with friends to organise some camping trips and it’s a pretty handy tool that saves on a lot of emails.

Alternatives -  Timebridge

OMERO –  OMERO is an microscopy image management tool created especially for scientists. It’s designed by the Open Microscopy Enviroment team which is based in multiple sites across the globe. Once you have an account set up and downloaded the programs, you can upload your images to a central server and process/analyse images and even make them ready for publication with a nice figure making tool. The Journal of Cell Biology has a data viewer based on OMERO that allows authors to upload images as they were acquired and users can look through z-stacks, time lapses and individual channels in these images.

If you have any tips for other useful freeware let us know in the comments section below.
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SDB Collaborative Resources (CoRe) Launched

Posted by on January 27th, 2012

The Society for Developmental Biology (SDB) has launched SDB Collaborative Resources (CoRe), an online collection of images, movies, and diagrams for learning and teaching developmental biology.  SDB CoRe is a free and open website developed to help increase understanding of developmental biology at all levels.




SDB CoRe is easily-searchable and can be browsed by topic, organism, or featured objects.  All objects have short descriptions aimed at helping users learn something about development with glossary words highlighted in green.  Object pages contain references as well as  links to related CoRe objects, links to reviews in the soon-to-be-launched WIREs Developmental Biology, and when relevant, to original research papers in SDB’s official journal Developmental Biology.  All users can create a My CoRe account in order to comment on an object or save it in their favorites.


SDB needs your help in building this community resource!  We are looking for visuals that help explain basic concepts in developmental biology across numerous plant and animal species.  Here are the guidelines for submitting to CoRe.  If you are an SDB member you can login to CoRe with your email address to submit.  Non-members that would like to submit to CoRe please contact me at info@sdbcore.org.  If you have any questions or suggestions for the site please email me as well.  Enjoy SDB CoRe!
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Bingo!

Posted by on January 9th, 2012

Somites, confocal, epigenetics, germline, stem cell… BINGO!

Thanks to all of your help and suggestions, BenchFly has now produced the Developmental Biology Group Meeting Bingo game.

From their post:

“Bingo? Are we actually suggesting you gamble during seminars? Yes! No. We’re simply providing a few key words that you may listen for during a talk… and if it just so happens that your card yields “Bingo!” sooner than your labmates’ and they have to take you out to lunch as a result, so be it…”

Visit BenchFly to play the game. You can refresh the card to get individual ones. There are over a hundred words, so there are many different possible cards to get. Good luck!
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Let’s make a developmental biology bingo game!

Posted by on December 5th, 2011

Ever noticed how each field has its own jargon?

Benchfly, a site with free video protocols and other resources for researchers, has created “Group Meeting Bingo”. The site generates bingo cards with the particular phrases common to various fields of research. They have cards for biochemistry, cell biology, and various other fields, but no developmental biology…yet!

So, let’s make a developmental biology bingo game!

Over the next few weeks (until we have enough words), you can leave a comment below (no registration required) with your suggestions for typical words that regularly show up in developmental biology talks. Benchfly will then turn our suggestions into a playable bingo game!

They suggest taking out the cards during meetings, but I’ve enjoyed just refreshing the existing cards on the site and marveling at all the field-specific words.


Section of one of the cell biology bingo cards. Of course some of the words from other fields can appear on the developmental biology cards as well!

Looking forward to see what you all come up with for the developmental biology game!

NB: The Node does not endorse playing bingo at the expense of paying attention to talks. Personally I’ve played a similar game at a conference where the meeting organizers handed out the cards, and encouraged everyone to play. I found it very easy to pay attention to the talks there, take notes, learn things, and still win the game. It’s actually easier to spot the words if you are paying attention!
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Site update and dates for your calendar

Posted by on September 15th, 2011

Scheduled Node Maintenance:

This weekend (September 16-18) we’re upgrading the system that the Node runs on (WordPress), so you may not be able to access the site at times. Everything should be working again on Monday, but as always, if you spot anything unusual, let us know.

Update 18/9: the site upgrade is now complete, and everything works - as far as we can tell (again, do let us know if something seems weird.)

Dates for your calendar
In the recent survey about the Node, a few people asked to be kept up to date of various scholarships and registration deadlines. Here is a selection of upcoming dates of interest, but this is by no means an exhaustive list. We’ll try to do these once in a while, but don’t hesitate to write your own posts to let people know about similar deadlines, or leave a comment below. Also make sure to check the eligibility of all scholarships and grants before applying.

Conference registration deadlines.
Keystone announced a few upcoming deadlines for conference abstract submissions, including dates for the following meetings:
September 19 – abstract & scholarship deadline for “Angiogenesis: Advances in Basic Science and Therapeutic Applications” (January 16-21, 2012)
September 20 – abstract & scholarship deadline for “Epigenomics” joint with “Chromatin Dynamics” (January 17-22, 2012)
September 21 – abstract & scholarship deadline for “Cardiovascular Development and Regeneration” (January 22-27, 2012)
October 6 - abstract & scholarship deadline for “Gene silencing by small RNAs” (February 7-12, 2012)

Grants and fellowships:
October 11 - The NSF announced an application deadline of October 11 for Postdoctoral Research Fellowships in Biology (PRFB). For 2012 this fellowship is limited to certain areas: (1) Broadening Participation in Biology; (2) Intersections of Biology and Mathematical and Physical Sciences; and (3) National Plant Genome Initiative Postdoctoral Research Fellowships.
November 1 – Sir Henry Wellcome PostDoctoral Fellowships. See their website for other grant deadlines.
November 18 – NSF Graduate Research Fellowship Program (GRFP)

Travel funding:
September 30 – Deadline for The Company of Biologists Direct Travel grants, which fund travel for conference attendance.
October 31 - EDEN has research exchange funds available for US-based eco-evo-devo researchers (graduate students, postdocs, faculty).
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Waves of early transcriptional activation and pluripotency program initiation during human preimplantation development

Posted by on August 9th, 2011

Since I was an undergraduate student at the Veterinary School in Milan, and throughout the rest of my scientific career, I have been fascinated with the complexities of mammalian preimplantation development. That’s why the publication of our recent paper “Waves of early transcriptional activation and pluripotency program initiation during human preimplantation development” feels like the natural conclusion of a long journey that started with buckets of cow ovaries in Italy, and ended with a collective effort shared by our team in the Stem Cell Bank at the Center for Regenerative Medicine in Barcelona (CMR[B]), under the direction of Drs Izpisua Belmonte and Veiga.

In just hours, the newly formed mammalian embryo terminates the program of the two gametes that formed it, escapes apoptosis, remodels its chromatin to a functional state, starts dividing, and turns on its genome while using up the reserves of protein and RNA inherited from mum (and dad, a little). This last process, termed embryonic genome activation (EGA), represents one of the first signs of “independent life” of a new individual.

As much as the researchers at the CMR[B] are used to manipulating embryos and work with tiny amount of material, studying preimplantation processes in general, and EGA in particular, is a great challenge in our species, as embryos are very scarce, heterogeneous in quality, and RNA amplification methods almost invariably introduce a very significant bias in downstream data quality. An incredible opportunity to look into details of this process came as the CMR[B] established a collaboration with Herbert Auer of the University of Barcelona. His facility just recently validated a method to amplify very small amount of RNA without bias, and we proceeded to apply this “pico-profiling” to a very detailed time course of single human embryos.

Among the many complex interactions that our study has unveiled, one certainly stands out: the human embryo starts EGA one full day before we thought since seminal works were published more than 30 years ago (Braude et al, 1988). Using a combination of extremely reliable transcriptional profiling and de novo transcription inhibition by amanitin treatment, we have been able to show that the human embryo transcribes from its own genome as early as the 2-cell stage (about 30hr after fertilization).

In order to make our data genome-wide expression data easily available to the scientific community at large we have prepared a free online database, HuMER (Human Embryo Resource; http://intranet.cmrb.eu/Human_embryos/home.html). It is our hope and desire that this resource would help us improve our ability to draw interdisciplinary connections between biological events and, in the process, increase our understanding of preimplantation development.

Written by first author of the paper, Dr. Rita Vassena.

ResearchBlogging.orgVassena, R., Boue, S., Gonzalez-Roca, E., Aran, B., Auer, H., Veiga, A., & Belmonte, J. (2011). Waves of early transcriptional activation and pluripotency program initiation during human preimplantation development Development, 138 (17), 3699-3709 DOI: 10.1242/dev.064741
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Map of Life: A guide to convergent evolution

Posted by on May 4th, 2011

The Map of Life is a recently published guide to convergent evolution produced by the University of Cambridge that has been touring science festivals and events across the world. It contains hundreds of article about structures and adaptations that have evolved independently in unrelated organisms such as camera eyes in jellyfish and snails to gliding in lizards and mammals.

This project is co-ordinated by the Professor of Evolutionary Palaeobiology, Simon Conway Morris in the Department of Earth Sciences. Professor Conway Morris and his team have spent several years on the project and the depth of the Map of Life really reflects this. The articles are all interlinked to each other, making it less of a list of convergent adaptations and more of a well linked database. I found myself wandering from camera eye evolution (they evolved 7 times!) to cognition in birds!

Map of  Life Homepage

All the information presented here comes from peer reviewed journals and other scientific literature. Although this seems primarily aimed at students and academics it is written in a way that also makes it accessible to members of the public (with some basic understanding of science).

The Map of Life does a great job in showing off the beauty (and laziness!) of evolution and how it arrives at the same or similar adaptations independently. It also tells us that evolution can be predictable when faced with similar environmental or selective pressures and interestingly, could also give us some clues about how life could evolve on other planets or moons.

The main message that the Map of Life presents is that evolution is true. Whilst there are may proponents of evolution, what really separates this message from others is that it is funded by the John Templeton Foundation, an organisation that aims to align science and religion. Whilst this organisation has been at the centre of controversy recently, it is quite refreshing to see them displaying the marvels of evolution with a resource that is so accessible and absorbing.

Links:

Map of Life - http://www.mapoflife.org
Map of Life on Facebook - http://www.facebook.com/mapoflife
Press release from Cambridge University - http://www.admin.cam.ac.uk/news/dp/2011030302
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The Cell: An Image Library featured on the Health and Human Services Recovery page

Posted by on April 18th, 2011

The Cell: An Image Library featured on the Health and Human Services Recovery page.


Cell Images Aid Researchers, Educators and the Public

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