the community site for and by developmental biologists

Displaying posts in the category: Resources [Clear Filter]

Pleiades Promoter Project

Posted by on September 27th, 2010

A recent paper in PNAS describes the development of MiniPromoters: human DNA promoters of less than 4 kb, designed to drive gene expression in specific areas of the brain. The initiative is called the Pleiades Promoter Project, and so far they have confirmed brain-region specific activity in knockin mice for 27 of their MiniPromoters. The[…]

Some tips for reading the Node

Posted by on July 14th, 2010

The Node has been officially live for almost four weeks now, and we’ve seen visitor numbers and registrations go steadily up. As with most websites, there are far more silent readers than people who are actively writing, so here are a few tips on keeping up to date with the Node: If you don’t want[…]

Protocol: Rehydrating embryos once fixed and dehydrated…

Posted by on July 9th, 2010

Does anyone know a protocol for rehydrating embryos once stored in methanol?  I’m working with Xenopus laevis embryos which have been fixed using formaldehyde.  Some lab wisdom passed down is “wash into a 50:50 solution of 1xPBS and methanol; then into 1xPBS; then into methanol again (which we’re a bit confused by); then into 50:50[…]

Teaching embryology to undergraduates

Posted by on June 18th, 2010

I am employed as a graduate teaching assistant for a first year biomedical sciences course at Auckland University in New Zealand. The class is called Cellular and Developmental Biology and my job is to help with tutoring the laboratory classes. For my lab streams I’m the one who stands at the front and gives instructions,[…]

Write your next article with your head in a cloud

Posted by on June 12th, 2010

There are many ways to write a collaborative research article, review, grant or conference proposal. One of the most comfortable frameworks when there are more than two of you actually doing the writing, and especially if you’re scattered on different continents, is Google Docs. Documents in progress such as your article text, abstract, cover letter[…]

Tips for blogging from meetings

Posted by on May 11th, 2010

At the Node we welcome reports from scientific meetings, but only if the meeting organizers and speakers allow it. I’m currently writing the Node’s guidelines for meeting reports, and then realized I could fill an entire post with additional information and links. Unlike the guidelines, anything in this post is very general advice and not[…]