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Displaying posts with the tag: gene-expression [Clear Filter]

Lighting Up the Central Dogma in Development

Posted by on June 19th, 2018

We recently published a manuscript in Cell that describes a method to image transcription factor concentration dynamics in real time, in living embryos, using a nanobody-based protein tag that we call the “LlamaTag.” We were particularly excited about these investigations because this new technology overcomes a major technical obstacle to understanding how gene-expression dynamics are[…]

Transposons in Embryo Space – TRACER maps in EMAGE

Posted by on February 9th, 2017

A recent publication in Developmental Biology by (Armit et al., 2017) describes how the TRACER dataset can be spatially compared with in situ hybridisation gene expression profiles.   The TRACER dataset of transposon-associated regulatory sensors (Chen et al., 2013) utilises Sleeping Beauty lacZ transposons that have been randomly integrated into the mouse genome Hundreds of[…]

New data reveals gene expression during mouse embryo development

Posted by on September 29th, 2016

This article was originally posted on the DMDD website dmdd.org.uk Knowing the ‘normal’ expression of genes during embryo development is key to understanding the differences that occur due to genetic mutations. As part of work to understand the underlying transcriptional processes for developing embryos from knockout mouse lines, DMDD has now released a gene expression[…]

An illustrated anatomical ontology of the developing mouse lower urogenital tract

Posted by on May 25th, 2015

Georgas et al. have presented a comprehensive update to the anatomical ontology of the murine urogenital system. These updates pertain to the lower urinary tract, genital tubercle and associated reproductive structures, covering stage E10.5 through to adult. The updates have been based on recently published insights into the cellular and gross anatomy of these structures,[…]

Report on GUDMAP Outreach at ISN World Congress of Nephrology (ISN WCN)

Posted by on March 30th, 2015

Report on GUDMAP Outreach at ISN World Congress of Nephrology (ISN WCN) March 13-17, 2015 Cape Town, South Africa Author: Chris Armit Date: 23rd March 2015 Introduction The International Society of Nephrology (ISN) holds biennial meetings throughout the world, and this was the first ISN WCN to be held in Africa. There was attention brought[…]

Of mice and men: exploring Mouse ENCODE

Posted by on December 17th, 2014

The Mouse ENCODE Project released a slew of papers late last month reporting findings from a three-year effort to comprehensively map functional elements in the mouse genome. Their major findings are summarized in an integrative paper in Nature (Yue, F. et. al., 2014). Similar to the goals of the human ENCODE project (The ENCODE Project Consortium,[…]

GUDMAP – an online resource for genitourinary research

Posted by on February 26th, 2014

GUDMAP (Harding et al., 2011, McMahon et al., 2008) is an open-access atlas-based on-line resource developed by a consortium of laboratories to provide the scientific and medical community with resources to facilitate research and teaching focussed on the murine genitourinary (GU) system. Gene expression data, descriptors of new mouse lines, and other tools can be[…]

Gene regulation and developmental biology at the Royal Society

Posted by on November 5th, 2012

Last week, the Royal Society hosted a meeting entitled “Regulation from a distance: Long-range control of gene expression in development and disease”. The impressive London offices of the Society (complete with double helix-inspired door handles) added a sense of occasion to what was bound to be a fascinating meeting, based on the list of excellent[…]

A wave from Quintay

Posted by on January 31st, 2012

The International course on Developmental Biology was a great experience, both instructive and mind-opening. All the students were shuttled to the remote and very small fishing village of Quintay, where the CIMARQ, the investigation centre where the course took place, is located. Originally a whaling station, this centre is dedicated to the instruction of professionals[…]

Visualize it!

Posted by on July 19th, 2011

Here’s a short “behind the scenes” on our paper about visualizing gene expression data, published in Nucleic Acids Research. When I joined James Briscoe‘s lab as a postdoc, my project’s ultimate goal was (and still is) to decipher and model the gene regulatory network underlying neural tube patterning. For this, I need to know where,[…]