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Displaying posts with the tag: mutagenesis [Clear Filter]

Sweetening with a pinch of salt: maximized Cas9 efficiency in zebrafish

Posted by on June 14th, 2016

  Alexa Burger, Mosimann lab, Institute of Molecular Life Sciences, University of Zürich, Switzerland. When I first heard about the “new” genome editing method in early 2013 called CRISPR-Cas9, I thought: “Never ever again will I work with targeted nucleases!” Now it’s mid-2016, we published our approaches to maximize Cas9 effectiveness in zebrafish with Development[…]

Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9

Posted by on October 31st, 2014

Researchers have always been interested in tissue-specific loss of function to probe the role of specific genes in embryonic development, cell physiology and disease conditions. Migration of lateral plate primordial germ cells in zebrafish, border cell migration during oogenesis in drosophila, interaction of T-cells with their target, and numerous other cases have continued to give[…]