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Protocol: Rehydrating embryos once fixed and dehydrated…

Posted by on July 9th, 2010

Does anyone know a protocol for rehydrating embryos once stored in methanol?  I’m working with Xenopus laevis embryos which have been fixed using formaldehyde.  Some lab wisdom passed down is “wash into a 50:50 solution of 1xPBS and methanol; then into 1xPBS; then into methanol again (which we’re a bit confused by); then into 50:50[…]

Write your next article with your head in a cloud

Posted by on June 12th, 2010

There are many ways to write a collaborative research article, review, grant or conference proposal. One of the most comfortable frameworks when there are more than two of you actually doing the writing, and especially if you’re scattered on different continents, is Google Docs. Documents in progress such as your article text, abstract, cover letter[…]