Thanks for your comment. That's a good point, and I wondered the same thing when I participated in the turtle research team for the first time. Actually, soft-shelled turtles have the genotypic sex-determining mechanism (ZZ/ZW type), unlike many other reptiles who have temperature-dependent sex-determining mechanism. So, it is expected that our embryos include both male and female individuals (though I have not checked the gender of each embryo/newborn).