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Journal club on the Node

Figure 1 Schematic of the clock model as proposed by Thorogood (1991). (A) The bold arrow represents the timing of the AER-to-AF transition in the developmental process. (B-D) Hypothesized representations of fin/limb development in the clock model (above) with endochondral skeletal patterns of the fin/limb (below,). (B) Fin development in a teleost, demonstrating a short period of time with AER signaling prior to the AER-to-AF transition.   (C) Fin development in lobe-finned fishes, showing a longer relative time with AER signaling prior to AF transformation. (D) Limb development in a tetrapod, in which AER signaling persists throughout limb development. Figure modified from Yano et al. [3]; based on Thorogood [2]; with fossil form representations in C-D from Long et al. [4].

If I could turn back time: an embryological look at the fin-to-limb transition

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Stem Cells in Developmental Biology: a debate at the BSDB

On how odd critters can answer important questions

Postdoc position

Posted by on January 4th, 2013

A postdoctoral position is available in the laboratory of Dr. Sophie Astrof to study roles of cell-extracellular matrix interactions in cardiovascular development and disease using mouse model system. The research will involve investigation of the role of extracellular matrix in orchestrating signaling/communication between various progenitor cell populations during morphogenesis of the aortic arch arteries.  In our lab, we use genetics, conditional mutagenesis, and transgenic approaches to explore roles of tissue microenvironment during organogenesis and disease. Experience with genetic manipulation, embryology and cell biology is desirable.  My laboratory is a part of the Center for Translational Medicine at Jefferson Medical College (http://www.tju.edu/jmc/medicine/translational_medicine/faculty/astrof.cfm?detail=0) located in the heart of Philadelphia. To apply, send a letter of interest, CV and names and contact information of three references to sophie.astrof@gmail.com

 

 
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Fast times at MBL

Posted by on June 19th, 2012

Sitting down to write this reflection, it struck me that I couldn’t believe it has really been two weeks since I arrived in the quiet town of Woods Hole, MA for the Embryology course at the Marine Biological Laboratory (MBL). Time has become an abstract concept for my fellow students and me; it seems like we just got here and yet have known one another for months. We engage in science almost every hour of every day. There is no work – only play. I was imaging immunostained plankton on a new Nikon A1 confocal microscope at 3am Saturday morning. I would have gone all night except I did not want to miss Nipam Patel’s famous butterfly lecture at 9am the same morning. Again, this isn’t work. I want to be doing this – and so does everyone else here.

I don’t know what generates the enthusiasm and energy at the MBL. In my graduate lab back at the University of Washington I am a hard worker, yet I don’t have the inclination (or ability) to maintain the pace that I have picked up here. It is not uncommon to work in lab until 2 or 3am, and yet we all eagerly attend lectures a few hours later and put in another 15+ hour day, six or seven days a week. Perhaps it’s the access to the best scientific equipment around – the sheer quantity of reagents and quality of microscopes available to us is stunning. However, more likely it’s being removed for six weeks from my regular graduate school environment. There is no pressure to generate actual data during my time here, no PI to disappoint, no lab meetings to prepare, and certainly no time to read current papers (I’ll have a lot of catching up to do in August!). There is only active experimentation. I am encouraged to ask my own questions and take ridiculous risks. I can do what interests me, even if there is no apparent face value for my field. For example, I spent last week honing my antibody staining skills by asking the question, “What is the prettiest combination of antibodies one can use on Drosophila embryos?” At the same time there is enough structure to ensure that I am learning the principles of developmental biology at an alarming pace.

I am a student of molecular biology but fairly new to the field of development, so I initially worried that I would struggle to keep up with students who were already experienced in developmental biology. These worries were unfounded as we all have as varied scientific backgrounds as our countries of origin. As one of five Americans in our class of twenty-four, this experience has allowed me to discover the commonalities in the language of science. Our techniques and questions might be different, but our curiosity and passion is matched. We were told that we would form bonds that will last the rest of our careers here, and two weeks in, I have little doubt that this was the truth.

When we are not actively manipulating embryos, world-renowned scientists distill their experience into our exhausted minds. We absorb as much as we can and then teach them drinking games in the wee hours of the morning. We have learned how to operate multitudes of imaging systems and practiced performing essential experimental techniques on more organisms than most people experience in a lifetime. In just two weeks we have been immersed in the history and current research directions of several models of development, including sea urchin, C. elegans, Drosophila, and many other invertebrates. Each model brings with it new opportunities and excitement, as well as traditions and challenges.

As we make the transition to vertebrate models, I am glad to take this moment to pause and reflect on my experiences so far. Six weeks is a long time to step out of one’s graduate research (and not to mention one’s life). It’s hard to assess what this time will mean to me in the years to come as I continue my own research. But if I were able to continue to engage as fully with the science of life as I have these last two weeks, I would never look back.

 



 

 

 
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Avian Model Systems 7: Nagoya Chick Meeting

Posted by on February 21st, 2012

 


 

After the devastating earthquake last year forced us to cancel the chick meeting, we are happy to announce that the next chick meeting will be held in Nagoya, Japan. The meeting will be held from 14th to 18th November 2012.


Please check the website for further details.

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Feed me!

Posted by on December 16th, 2010

With people in many countries preparing to take a few days off at the end of this month, and other countries starting their summer break, I’m sure many of you have had to deal with the stress of handling your experiments over the holidays. How do you explain to a tank of zebrafish or a flask of cells that you’re going to visit your family for a few days? Do you just not go at all? Is the whole lab leaving feeding instructions with that new postdoc who lives just across the street from the institute and comes in every day anyway?

Let us know via the poll, and leave a comment if you want to explain your answer.

(poll closed and archived)


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