![Figure 1 Schematic of the clock model as proposed by Thorogood (1991). (A) The bold arrow represents the timing of the AER-to-AF transition in the developmental process. (B-D) Hypothesized representations of fin/limb development in the clock model (above) with endochondral skeletal patterns of the fin/limb (below,). (B) Fin development in a teleost, demonstrating a short period of time with AER signaling prior to the AER-to-AF transition. (C) Fin development in lobe-finned fishes, showing a longer relative time with AER signaling prior to AF transformation. (D) Limb development in a tetrapod, in which AER signaling persists throughout limb development. Figure modified from Yano et al. [3]; based on Thorogood [2]; with fossil form representations in C-D from Long et al. [4].](http://thenode.biologists.com/wp-content/uploads/2013/04/node1-70x45.png){kind=small}
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1. Widefield image of a pilidium larvae of the Nemertean ribbon worm, Cerebratulus lacteus, stained for F-actin (green; phalloidin), Acetylated tubulin (red) and DAPI (blue; nuclei)
2. Confocal image of Crepidula fornicata (slipper limpet) embryo stained for FMRF (yellow), Acetylated tubulin (green) F-actin (purple; phalloidin) and DAPI (blue; nuclei)
3. Confocal image of squid, Loligo pealei, embryo stained for for F-actin (green; phalloidin), Acetylated tubulin (red), anti-HRP (yellow), and DAPI (blue; nuclei)
4. Confocal image of squid, Loligo pealei, embryo stained for for F-actin (red; phalloidin), Acetylated tubulin (green), and DAPI (blue; nuclei)
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Also, beautiful images, especially number 2.
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I think 1 is well balanced and displays a wide range of colors while accentuating the underlying structures and evoking the feeling that the image is of a mysterious but living thing.
All the images however, are amazing and convey the complexity and beauty of life. Plus they are super-awesome (tm).
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