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About: Joana Lima

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Stable and bicistronic expression of two genes in somite- and lateral plate-derived tissues to study chick limb development

Posted by on November 27th, 2015

The electroporation technique is widely used in developmental biology to deliver foreign DNA into cells and study gene function. The chick embryos exhibit a remarkable easy access to perform electroporation and follow in ovo development. Electroporation of limb somites allows the misexpression of genes in limb somite derivatives, like myogenic and endothelial cells, while electroporation[…]