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developmental and stem cell biologists

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 140, Issue 10)

Posted by , on 30 April 2013

Here are the highlights from the current issue of Development:   In-cyst-ing on germ cell development During gametogenesis in many organisms, germ cells undergo synchronous, incomplete divisions just before meiosis ...

In Development this week (Vol. 140, Issue 9)

Posted by , on 9 April 2013

Here are the highlights from the current issue of Development:   Dynamics of human thymus development The thymus is the primary organ responsible for generating T cells. Although thymus development ...

In Development this week (Vol. 140, Issue 8)

Posted by , on 26 March 2013

Here are the highlights from the new issue of Development: Molecular map of posterior hypothalamus The hypothalamus is a key integrative centre in the vertebrate brain that regulates many essential ...

In Development this week (Vol. 140, Issue 7)

Posted by , on 12 March 2013

Here are the highlights from the current issue of Development:   Mammary gland RankL-ed into making milk Extensive remodelling of the mammary gland during pregnancy generates milk-producing lobuloalveolar structures. During ...

In Development this week (Vol. 140, Issue 6)

Posted by , on 27 February 2013

Here are the highlights from the current issue of Development:   Cofilin and Vangl2 kick start planar cell polarity The planar cell polarity (PCP) pathway orients cells within the plane ...

Recent comments by Seema Grewal

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps
by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010