Seema Grewal, Author at the Node

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 140, Issue 16)

Posted by Seema Grewal, on 30 July 2013

Here are the highlights form the current issue of Development: Nf2 regulates neural progenitor proliferation Mutation of neurofibromatosis 2 (NF2) results in nervous system tumours. Molecularly, Nf2 has diverse ...

In Development this week, Vol. 140, Issue 15

Posted by Seema Grewal, on 16 July 2013

Here are the highlights from the current issue of Development: From hPSC to mature hepatocyte Liver development entails induction of definitive endoderm, specification of the endoderm to a hepatic ...

In Development this week (Vol. 140, Issue 14)

Posted by Seema Grewal, on 3 July 2013

Here are the highlights from the latest issue of Development. Extrinsic cue for dendrite polarisation Most neurons have a single axon on one side of their cell body and multiple ...

In Development this week (Vol. 140, Issue 13)

Posted by Seema Grewal, on 11 June 2013

Here are the highlights from the current issue of Development: Centrosomes and cell fate: a Notch ahead Asymmetric cell divisions (ACDs) play a crucial role in controlling cell fate ...

In Development this week (Vol. 140, Issue 11)

Posted by Seema Grewal, on 14 May 2013

Here are the highlights from the new issue of Development: Sniffing out sensory maps The development of sensory maps – neural representations of the sense organs – involves the ...

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps

by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010