Seema Grewal, Author at the Node

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 139, Issue 24)

Posted by Seema Grewal, on 21 November 2012

Here are the highlights from the current issue of Development: Translating Apc1 loss into intestinal proliferation Most colorectal cancers carry inactivating mutations in adenomatous polyposis coli (APC), a negative ...

In Development this week (Vol. 139, Issue 23)

Posted by Seema Grewal, on 6 November 2012

Here are the highlights from the current issue of Development: Dishevelled: the Notch-Wnt go-between The Notch and Wnt signalling pathways are used during animal development to generate a diverse ...

In Development this week (Vol. 139, Issue 22)

Posted by Seema Grewal, on 23 October 2012

Here are the research highlights from the current issue of Development: Nodal regulates germ cell potency During mammalian gonadal development, somatic cues regulate the sex-specific development of foetal germ ...

In Development this week (Vol. 139, Issue 20)

Posted by Seema Grewal, on 18 September 2012

Here are the highlights from the current issue of Development: S1P1 halts vascular remodelling During organogenesis, vascular remodelling ensures that developing organs receive sufficient oxygen and nutrients. Factors that ...

In Development this week (Vol. 139, Issue 19)

Posted by Seema Grewal, on 4 September 2012

Here are the highlights from the current issue of Development: Stem cells get cut loose During tissue maintenance and regeneration, stem cells (SCs) are mobilised and migrate towards sites of ...

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps

by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010