Seema Grewal, Author at the Node

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 140, Issue 5)

Posted by Seema Grewal, on 12 February 2013

Here are the research highlights from the current issue of Development: Evolution of mesoderm induction Fibroblast growth factors (FGFs) are essential for mesoderm induction in vertebrates and for early ...

In Development this week (Vol. 140, Issue 4)

Posted by Seema Grewal, on 29 January 2013

Here are the highlights from the current issue of Development: Pancreatic injury unlocks cell potential Identifying methods by which pancreatic β-cells can be produced is of major therapeutic importance. ...

In Development this week (Vol. 140, Issue 3)

Posted by Seema Grewal, on 8 January 2013

Here are the research highlights from the current issue of Development: Directed differentiation: Nodal steps forward The directed differentiation of pluripotent stem cells into endodermal derivatives, including insulin-producing pancreatic ...

In Development this week (Vol. 140, Issue 2)

Posted by Seema Grewal, on 18 December 2012

Here are the highlights from the current issue of Development: Against the segmentation clock A gene expression oscillator called the segmentation clock controls the periodic formation of somites in ...

In Development this week (Vol. 140, Issue 1)

Posted by Seema Grewal, on 4 December 2012

Here are the highlights from the current issue of Development: Gut feeling about Wnt doses The intestinal epithelium continuously renews throughout life. Canonical Wnt signalling – a major player ...

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps

by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010