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developmental and stem cell biologists

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 139, Issue 8)

Posted by , on 20 March 2012

Here are the highlights from the current issue of Development: Chewing the Fat over PCP and growth The Drosophila protocadherin Fat (Ft) affects planar cell polarity (PCP) but also inhibits ...

In Development this week (Vol. 139, Issue 7)

Posted by , on 6 March 2012

Here are the research highlights from the current issue of Development: miR-125 seals hESC neural fate MicroRNAs, small non-coding RNAs, have recently emerged as key regulators of embryonic development. In ...

In Development this week (Vol. 139, Issue 6)

Posted by , on 21 February 2012

Here are the research highlights from the current issue of Development: Neural circuit building During development, sensory neurons form neural circuits with motoneurons. Although the anatomical details of these circuits ...

In Development this week (Vol. 139, Issue 5)

Posted by , on 8 February 2012

Here are the highlights from the current issue of Development: ROCKed to the heart Noonan syndrome – a common cause of congenital heart disease – is often associated with missense ...

In Development this week (Vol. 139, Issue 4)

Posted by , on 24 January 2012

Here are the highlights from the current issue of Development: Neuronal cell fate: windows of opportunity It is becoming increasingly evident that both vertebrate and invertebrate neural progenitor cells exhibit ...

Recent comments by Seema Grewal

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps
by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010