Seema Grewal, Author at the Node

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 139, Issue 13)

Posted by Seema Grewal, on 6 June 2012

Here are the highlights from the current issue of Development: Two-step loss of pluripotency During early development, embryonic cells can form derivatives of all three embryonic layers. This pluripotency, which ...

In Development this week (Vol. 139, Issue 12)

Posted by Seema Grewal, on 22 May 2012

Here are the highlights from the current issue of Development: Mechanical changes in cochlea development Correct patterning of the mammalian inner ear sensory epithelium, which contains mechanosensory outer hair ...

In Development this week (Vol. 139, Issue 11)

Posted by Seema Grewal, on 8 May 2012

Here are the research highlights from the current issue of Development: Laminin cue for epithelial polarity During the development of many animal organs, mesenchymal cells co-ordinately polarize to form epithelial ...

In Development this week (Vol. 139, Issue 10)

Posted by Seema Grewal, on 23 April 2012

Here are the highlights from the current issue of Development: A TOR de force in the haematopoietic niche During development and homeostasis, it is essential to coordinate growth with the ...

In Development this week (Vol. 139, Issue 9)

Posted by Seema Grewal, on 3 April 2012

Here are the highlights from the current issue of Development: Haematopoiesis to knock your SOX7 off During vertebrate development, haematopoietic and endothelial cells emerge from a common mesodermal progenitor, ...

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps

by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010