The community site for and by
developmental and stem cell biologists

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 138, Issue 7)

Posted by , on 8 March 2011

Here are the research highlights from the current issue of Development: A breath of fresh air: miRNAs regulate lung development Throughout development, a proper balance between the proliferation and differentiation ...

In Development this week (Vol. 138, Issue 6)

Posted by , on 22 February 2011

Here are the research highlights from the current issue of Development: Arteriovenous-specific regulation of angiogenesis Endothelial cells (ECs) assume arterial- or venous-specific molecular characteristics at early stages of development. These ...

In Development this week (Vol. 138, Issue 5)

Posted by , on 8 February 2011

Here are the highlights from the current issue of Development: From pluripotent to pancreatic fates A reliable method for generating insulin-producing β-cells from human pluripotent stem cells (hPSCs) would provide ...

In Development this week (Vol. 138, Issue 4)

Posted by , on 26 January 2011

Here are the research highlights from the current issue of Development: New moves in haematopoiesis: rumba and samba Vertebrate haematopoiesis relies on a pool of haemetopoietic stem/progenitor cells (HSPCs) that ...

In Development this week (Vol. 138, Issue 3)

Posted by , on 4 January 2011

Here are the research highlights from the new issue of Development: Wnt/PCP signalling, microtubules and gastrulation During vertebrate gastrulation, convergence and extension (C&E) movements shape the germ layers to form ...

Recent comments by Seema Grewal

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps
by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010