Seema Grewal, Author at the Node

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 138, Issue 12)

Posted by Seema Grewal, on 24 May 2011

Here are the highlights from the current issue of Development: How transcriptional silencing goes into reverse The Polycomb group (PcG) machinery silences terminal differentiation genes in stem cell lineages. Reversal ...

In Development this week (Vol. 138, Issue 11)

Posted by Seema Grewal, on 10 May 2011

Here are the highlights from the current issue of Development: Dronc regulates Numb and neuroblast formation The ability of stem cells to maintain a balance between self-renewal and differentiation is ...

In Development this week (Vol. 138, Issue 10)

Posted by Seema Grewal, on 26 April 2011

Here are the research highlights from the current issue of Development: FatJ keeps neural progenitor pools in shape The correct development and functioning of the spinal cord depends on the ...

In Development this week (Vol. 138, Issue 9)

Posted by Seema Grewal, on 12 April 2011

Here are the research highlights from the current issue of Development: Cranial neural crest development: p53 faces up The tumour suppressor p53 plays multiple roles in the prevention of cancer ...

In Development this week (Vol. 138, Issue 8):

Posted by Seema Grewal, on 22 March 2011

Here are the research highlights from the current issue of Development: Fishing out adult neural stem cells Adult neural stem cells (NSCs) hold great potential for the treatment of neurodegenerative ...

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps

by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010