Cold Spring Harbor Laboratory Cell & Developmental Biology of Xenopus: Gene Discovery & Disease

Gene manipulation

·     CRISPR/Cas9 knock-out

·     Antisense Morpholino knock-down

·     mRNA and DNA over-expression

·     Small molecule drug treatment

·     Transgenesis

Experimental Techniques

·     Targeted microinjections

·     Tissue transplantation

·     Organoid generation

·     Dissections and ex vivo organ culture

·     Biomechanics

·     Single cell technologies

Analysis Methods

·     Immuno-fluorescence and labeling

·     mRNA in situ hybridization and hybridization chain reaction (HCR)

·     Bioinformatics and imaging analysis

·     High-resolution confocal, spinning-disc and 2-photon microscopy

·     Time-lapse and live-cell imaging

·     Plate-based imaging and optical coherence tomography