Research technician post in stem cell/cancer biology (University of Sheffield, UK)
Posted by Anestis Tsakiridis, on 10 July 2019
Closing Date: 15 March 2021
We are looking to appoint a Research Technician who will provide support to a one-year Neuroblastoma UK-funded project entitled “Establishment of an in vitro model of neuroblastoma initiation using pluripotent stem cell differentiation”. The project aims to dissect the cellular and molecular basis of neuroblastoma initiation using human pluripotent stem cell (hPSCs) differentiation and hPSC lines engineered to ectopically overexpress common neuroblastoma-associated oncogenes.
You will join a research team under the guidance of Dr Anestis Tsakiridis, providing support for routine hPSC culture and differentiation, preparation of samples/analysis and carrying out molecular cloning/genetic modification of hPSCs. Appropriate training will be provided. This is an excellent opportunity to gain hands-on laboratory experience and to be part of a leading research team. Our group’s research aims to define the molecular basis of cell fate decisions during human embryonic development and determine how “altered” embryonic multipotent states drive tumourigenesis (https://www.tsakiridislab.com/).
Applicants must have a good honours degree or equivalent experience in a developmental/stem cell biology-related subject along with previous experience of working in a research laboratory. Familiarity with some/all of the following techniques is desirable: hPSC culture; RNA isolation; molecular cloning; mammalian cell transfection; quantitative real time PCR; immunostaining; fluorescence microscopy; flow cytometry. Applicants should also have an interest in stem cell and developmental biology.
To apply: visit the University of Sheffield job portal (https://www.sheffield.ac.uk/jobs/index)
Closing date: 7th August 2019
Expected start date: 1st September 2019
For more details/questions contact: a.tsakiridis@sheffield.ac.uk