Seema Grewal, Author at the Node

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 140, Issue 21)

Posted by Seema Grewal, on 15 October 2013

Here are the highlights from the current issue of Development: Deconstructing pancreas development in vitro An effective cellular therapy for diabetes is dependent on the production of sufficient quantities ...

In Development this week (Vol. 140, Issue 20)

Posted by Seema Grewal, on 1 October 2013

Here are the highlights from the new issue of Development: Coordinating stem cell and niche development The Drosophila ovary provides an accessible system for analysing the interactions between stem ...

In Development this week (Vol. 140, Issue 19)

Posted by Seema Grewal, on 17 September 2013

Here are the highlights from the new issue of Development: Roadmap for human trophoblast model Correct trophoblast development is essential for placenta function and embryogenesis. In humans, the early ...

In Development this week (Vol. 140, Issue 18)

Posted by Seema Grewal, on 27 August 2013

Here are the highlights from the new issue of Development: FGF10 function in the lung branches off Lung development in mice involves specification of the primary lung field followed ...

In Development this week (Vol. 140, Issue 17)

Posted by Seema Grewal, on 13 August 2013

Here are the highlights from the current issue of Development: GDNF’s got a nerve Innervation of the mammalian pancreas is crucial for endocrine and exocrine function. Neural crest cells that ...

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps

by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010