Seema Grewal, Author at the Node

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 139, Issue 18)

Posted by Seema Grewal, on 21 August 2012

Here are the highlights from the current issue of Development: Retinoic acid’s developmental role illuminated The role of all-trans retinoic acid (RA) in hindbrain development and other developmental processes ...

In Development this week (Vol. 139, Issue 17)

Posted by Seema Grewal, on 7 August 2012

Here are the highlights from the current issue of Development: eIF4E-3 puts a cap on spermatogenesis Gene expression is translationally regulated during many developmental processes. Translation is mainly controlled ...

In Development this week (Vol. 139, Issue 16)

Posted by Seema Grewal, on 24 July 2012

Here are the research highlights from the current issue of Development: Less air, more muscle repair Oxygen levels in stem cell niches are often lower than those in surrounding ...

In Development this week (Vol. 139, Issue 15)

Posted by Seema Grewal, on 10 July 2012

Here are the research highlights from the current issue from Development: Cxcr4a sets proliferative response to Hh The Hedgehog (Hh) pathway controls both patterning and proliferation during development, but ...

In Development this week (Vol. 139, Issue 14)

Posted by Seema Grewal, on 26 June 2012

Here are the highlights from the current issue of Development: BRAF signalling sets astrocyte numbers Astrocytes are the most numerous cell type in the mammalian central nervous system but little ...

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps

by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010