Seema Grewal, Author at the Node

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 139, Issue 3)

Posted by Seema Grewal, on 12 January 2012

Here are the highlights from the current issue of Development: CycA takes control of endoreplication Endocycles – repeated rounds of DNA replication without intervening mitoses – are involved in ...

In Development this week (Vol. 139, Issue 2)

Posted by Seema Grewal, on 20 December 2011

Here are the highlights from the current issue of Development: Balanced ephrin/Eph signals drive topographic mapping During development of the retinotectal axonal projection, which connects the retina to the ...

In Development this week (Vol. 139, Issue 1)

Posted by Seema Grewal, on 6 December 2011

Here are the highlights from the current issue of Development: Notching up pancreas development According to the lateral inhibition model, during early pancreas development, Neurog3 expression in multipotent progenitor cells ...

In Development this week (Vol. 138, Issue 24)

Posted by Seema Grewal, on 22 November 2011

Here are the research highlights from the current issue of Development: Getting to the heart of Flk1 expression The Flk1 gene, which encodes a VEGF-A receptor, is expressed in the ...

In Development this week (Vol. 138, Issue 23)

Posted by Seema Grewal, on 8 November 2011

Here are the highlights from the current issue of Development: Skin-deep dermal niches Hair follicle formation in the epidermis depends on signals from the underlying dermis and normally only occurs ...

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps

by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010