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developmental and stem cell biologists

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 137, Issue 21)

Posted by , on 12 October 2010

Here are the research highlights from the current issue of Development: Oct1: essential for trophoblast development Most POU family transcription factors are temporally and spatially restricted during development and play ...

In Development this week (Vol 137, Issue 20)

Posted by , on 28 September 2010

A fateful look at early mouse lineage specification The first cell lineages specified in the mouse embryo are the trophectoderm (TE), which generates the embryonic portion of the placenta, and ...

In Development this week (Vol 137, Issue 19)

Posted by , on 7 September 2010

Here are the research highlights from the current issue of Development: Nr5a receptors reset EpiSC pluripotency Rodent embryonic stem (ES) cells that are derived from blastocysts self-renew without mitogenic growth ...

In Development this week (Volume 137, Issue 18)

Posted by , on 24 August 2010

Non-muscle myosin II translates cilia polarity In the brain, cilia on the multiciliated ependymal cells that line the brain ventricles circulate cerebrospinal fluid over the brain surface. To generate this ...

In the new issue of Development, 137 (17)...

Posted by , on 10 August 2010

The Bicoid gradient, epigenetic control of BMP signalling, haematopoietic stem cells and more…here are the highlights from the current issue of Development: The Bicoid gradient gets into shape without nuclei ...

Recent comments by Seema Grewal

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps
by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010