Post-doctoral position – repetitive DNA in somatic tissue homeostasis

Project:

Our team is interested in the role that non-genic, repetitive DNA sequences play in the maintenance of cellular homeostasis in somatic tissues. More specifically, an ERC-funded project will illuminate the modes of regulation and the impact of transposable elements (TEs) in the soma. TEs are omnipresent, self-propagating DNA sequences. Their expression and/or mobility are implicated in normal development, aging and pathologic conditions, such as cancer or neurodegenerative diseases.

Our primary model system is the Drosophila midgut, a self-renewing tissue maintained by a population of adult intestinal stem cells. We combine diverse techniques, such as Drosophila genetics, CRISPR/Cas9 genome/epigenome editing, microscopy, physiological assays, molecular biology and genomics (including short- and long-read sequencing).

Position:

Post-doctoral funding is available for up to 3 years. Starting date is flexible (from June 2023).

Requirements:

• Enthusiastic, curiosity-driven researchers are encouraged to apply
• You should hold (or expect to obtain) a PhD in cell biology, developmental biology or genomics
• Collaborative spirit and good level of spoken and written English are required
• Experience in at least one of the following areas is desired: Drosophila handling and genetics, NGS wet-lab/data analysis, transposable elements, light microscopy

Environment:

You will join a young, ATIP-Avenir-labeled and ERC Starting Grant-funded team, with permanent staff assisting in the implementation of the project. We are located in the Institute for Integrative Biology of the Cell (I2BC) in Gif-sur-Yvette, within a large CNRS/University Paris-Saclay science campus, in the south suburbs of Paris. The I2BC and the campus offer direct access to excellent facilities, training options, as well as support for international researchers (more info). The campus is conveniently connected to Paris, with its great scientific community and cultural life.

Contact:

For additional information, please email katarzyna.siudeja@i2bc.paris-saclay.fr

To apply, send your CV, cover letter and contact details of 2 referees in a single pdf file.

Please apply before May 31, 2023. However, note that applications will be reviewed as received. Candidates are encouraged to apply as soon as possible.

Key publications:

• Van den Beek M, Rubanova N, Siudeja K. Experimental Approaches to Study Somatic Transposition in Drosophila Using Whole-Genome DNA Sequencing. Methods Mol Biol. 2023;2607:311-327.
• Siudeja K, van den Beek M, Riddiford N, (…), Bardin AJ. Unravelling the features of somatic transposition in the Drosophila intestine. The EMBO Journal. 26 févr 2021;n/a(n/a):e106388.
• Riddiford N, Siudeja K, van den Beek M, (…), Bardin AJ. Evolution and genomic signatures of spontaneous somatic mutation in Drosophila intestinal stem cells. Genome Res. 24 juin 2021;gr.268441.120.
• Siudeja K, Nassari S, Gervais L, (…), Bardin AJ. Frequent Somatic Mutation in Adult Intestinal Stem Cells Drives Neoplasia and Genetic Mosaicism during Aging. Cell Stem Cell. 3 déc 2015;17(6):663‑74