Senior Research Technician in Human Stem Cell Biology

The Theunissen Lab in the Department of Developmental Biology and Center of Regenerative Medicine at Washington University in St. Louis (theunissenlab.wustl.edu) investigates the molecular mechanisms regulating pluripotent stem cells and their applications in regenerative medicine. A major focus of our work is to define the signaling pathways that govern the self-renewal and differentiation of distinct human pluripotent stem cell states and to establish novel cellular models of early human development and disease. We are currently seeking to recruit a highly motivated Senior Research Technician to assist with the technical aspects of our studies including both tissue culture and molecular biology. The primary focus of the work includes but is not limited to the following:

• Mammalian tissue culture, including culture of human embryonic stem cells (hESCs), induced pluripotent stem cells (iPSCs), and human trophoblast stem cells (hTSCs)
• Molecular biology techniques, including DNA, RNA, and protein isolation, quantitative RT-PCR analysis, and western blotting
• Immunohistochemistry and fluorescence microscopy
• Preparation of critical reagents, maintenance of general lab supplies, and ensuring that equipment is properly cleaned and maintained in accordance with established procedures
• Implementation and supervision of laboratory safety procedures

Candidates with a Bachelor’s degree in biology or a related discipline and several years of experience in a relevant laboratory setting are encouraged to apply. The qualified candidate should have strong troubleshooting skills, an ability to maintain complete and organized records, and be able to communicate effectively with the supervisor as well as other researchers.

To apply for this position please submit a CV, a cover letter describing your qualifications, and contact information for two references to t.theunissen@wustl.edu. Applications will be reviewed promptly until the position is filled. Washington University is an equal opportunity employer and complies with applicable EEO and affirmative action regulations.