March in preprints
Posted by the Node, on 4 April 2025
Welcome to our monthly trawl for developmental and stem cell biology (and related) preprints.
The preprints this month are hosted on bioRxiv and arXiv – use these links below to get to the section you want:
- Patterning & signalling
- Morphogenesis & mechanics
- Genes & genomes
- Stem cells, regeneration & disease modelling
- Plant development
- Environment, evolution and development
Research practice and education
Developmental biology
| Patterning & signalling
Wesley Chan, Justin Evans, Tony Roenspies, Jonathan D. Rumley, John I. Murray, Antonio Colavita
Alice M. Godden, Nicole Ward, Méghane Sittewelle, Marco Antonaci, Aleksandr Kotov, Anne H. Monsoro-Burq, Grant N. Wheeler
FGF diffusion is required for directed migration of postembryonic muscle progenitors in C. elegans
Theresa V Gibney, Ariel M Pani
Marco Mundaca-Escobar, Renato V. Pardo, Rodrigo E. Cepeda, Andres F. Sarrazin
DIO3 coordinates photoreceptor development timing and fate stability in human retinal organoids
Christina McNerney, Clayton P. Santiago, Kiara C. Eldred, Ian Glass, Tom A. Reh, Arturo Hernandez, Seth Blackshaw, Nathan D. Lord, Robert J. Johnston Jr
Cell polarity of neural crest-derived mesenchymal cells controls craniofacial development
Andrea Burianova, Kleopatra Kythraiotou, Lucie Sazavska, Ondrej Machon
Axial patterning of gastruloids via diverse cell type compositions
Kerim Anlas, Fumio Nakaki, Nicola Gritti, Jordi Font-Reverter, Krisztina Arató, Martí Planasdemunt-Hospital, David Oriola, James Sharpe, Vikas Trivedi
| Morphogenesis & mechanics
Katharine Goodwin, Theresa Anne Emrich, Sebastian Arnold, Katie McDole

Adaptive Clonal Expansion Shapes Brain Development
Giulia Di Muzio, Sarah Benedetto, Li-Chin Wang, Lea Weber, Franciscus van der Hoeven, Brittney Armstrong, Hsin-Jui Lu, Jana Berlanda, Verena Körber, Nina Claudino, Michelle Krogemann, Thomas Höfer, Pei-Chi Wei
Mechanical fracturing of the extracellular matrix patterns the vertebrate heart
Christopher Chan Jin Ji, Daniel Santos-Olivan, Marie-Christine Ramel, Juliana Sanchez-Posada, Priscilla Paizakis, Toby GR Andrews, Emily S Noel, Alejandro Torres-Sanchez, Rashmi Priya
Characterisation of the Avascular Mesenchyme during Digit Outgrowth
Cameron C Batho-Samblas, Jonathan Smith, Lois Keavey, Noah Clancy, Lynn McTeir, Megan G Davey
Adhesion to a common ECM mediates interdependence in tissue morphogenesis in Drosophila
LE Sánchez-Cisneros, M Barrera-Velázquez, Dimitri Kromm, P Bun, H Merchant-Larios, LD Ríos-Barrera
Coro1A and TRIM67 collaborate in netrin-dependent neuronal morphogenesis
Chris T. Ho, Elliot B. Evans, Kimberly Lukasik, Ellen C. O’Shaughnessy, Aneri Shah, Chih-Hsuan Hsu, Brenda Temple, James E. Bear, Stephanie L. Gupton
PIEZO1 Drives Trophoblast Fusion and Placental Development
Yang Zhang, Ke Z. Shan, Pengfei Liang, Augustus J. Lowry, Liping Feng, Huanghe Yang
Crucial roles of mesenchymal Gata2 in murine epididymal development
Allyssa Fogarty, Shuai Jia, Jillian Wilbourne, Claire DuPuis, Fei Zhao
| Genes & genomes
Ruth Klafke, Natalie Harriman, Richard J.T. Wingate, Andrea Wizenmann
Chronic replication stress-mediated genomic instability disrupts placenta development in mice
Mumingjiang Munisha, Rui Huang, John C. Schimenti
Binbin Ma, Guanghui Yang, Jonathan Yao, Charles Wu, Jean Pinckney Vega, Gabriel Manske, Saher Sue Hammoud, Satrajit Sinha, Abhyudai Singh, Haiqing Zhao, Xin Chen

Sex-biased Transcriptome in in vitro Produced Bovine Early Embryos
Meihong Shi, Guangsheng Li, Hannah Marie Araujo, Angie S Lee, Jingzhi Zhang, Yoke Lee Lee, IISAGE Consortium, Soon Hon Cheong, Jingyue (Ellie) Duan
Yarden Yesharim, Ophir Shwarzbard, Jenny Barboy-Smoliarenko, Ran Shachar, Amrutha Palavalli, Hanh Thi-Kim Vu, Schraga Schwartz, Omri Wurtzel
Developmental regulation of Drosophila dosage compensation in a 3D genome context
Lubna Younas, Mujahid Ali, Xinpei Zhang, Catherine Regnard, Qi Zhou
Zsombor Varga, Ferenc Kagan, Shingo Maegawa, Ágnes Nagy, Javan Okendo, Shawn M. Burgess, Eric S. Weinberg, Máté Varga
EV O’Neil, SM Dupont, B Capel
Hao Deng, Phillippe Valenti, Francois Payre, Bomyi Lim
Maternal Effects on Postembryonic Neuroblast Migration in C. elegans
Hoikiu Poon, Chaogu Zheng
Calcium signals shape metabolic control of H3K27ac and H3K18la to regulate EGA
Virginia Savy, Paula Stein, Don Delker, Martín A. Estermann, Brian N. Papas, Zongli Xu, Lenka Radonova, Carmen J. Williams
TENT5C extends Odf1 poly(A) tail to sustain sperm morphogenesis and fertility
Marine Baptissart, Ankit Gupta, Alexander C. Poirot, Brian N. Papas, Marcos Morgan
Dongni Shao Chen, Jin Man, Xian Shu, Haoer Shi, Xue Xia, Yusanjiang Abula, Yuu Kimata
Alyssa B. Long, Isabella M. Wilson, Tiffany T. Terry, Robert E. Van Sciver, Tamara Caspary

Annemarie J. Carver, Faith M. Fairbairn, Robert J. Taylor, Shanmukh Boggarapu, Njenga R. Kamau, Amrita Gajmer, Hanna E. Stevens
The pioneer factor Zelda induces male-to-female somatic sex reversal in adult tissues
Sneh Harsh, Hsiao-Yun Liu, Pradeep Kumar Bhaskar, Christine Rushlow, Erika Bach
| Stem cells, regeneration & disease modelling
Ziadoon Al-Akashi, Denise Zujur, Nicholas Boyd-Gibbins, Nathalie Eileen Wiguna, Masato Nakagawa, Tetsuhiro Kikuchi, Asuka Morizane, Jun Takahashi, Makoto Ikeya
Detection of dedifferentiated stem cells in Drosophila testis
Muhammed Burak Bener, Boris Slepchenko, Mayu Inaba
Extracellular matrix deposition controls early differentiation patterns in human cardiac gastruloids
Marion F Marchand, Geetika Sahni, Aditya Arora, Selma Sehrouchni, Florain Dilasser, Anais Monet, Flora Luciani, Harini Rajendiran, Lea Chabot, Saburnisha Binte Mohamad Raffi, Gianluca Grenci, Jean-Baptiste Sibarita, Remi Galland, Jin Zhu, Virgile Viasnoff
Small molecule influence on Caudal fin regeneration in Zebrafish: A proteomic based study
Anusha P V, Shailvi Tewari, Catherine Philip, Anushka Arvind, Ifra Iyoob, Mohammed M Idris
Tsz Long Chu, Ostap Dregval, Farasat Zaman, Lei Li, Xin Tian, Xin Liu, Dana Trompet, Baoyi Zhou, Jussi O Heinonen, Claes Ohlsson, Lars Sävendahl, Igor Adameyko, Andrei S Chagin
map3k1 is required for spatial restriction of progenitor differentiation in planarians
Bryanna Isela-Inez Canales, Hunter O. King, Peter W. Reddien

The Notch ligand Jagged1 plays a dual role in cochlear hair cell regeneration
Xiao-Jun Li, Charles Morgan, Lin Li, Wan-Yu Zhang, Elena Chrysostomou, Angelika Doetzlhofer
Defining the role of fibroblasts in skin expansion
Caroline Aguilera Stewart, Ceyhun Alar, Leslie Bargsted, Ioanna Kaklamanou, Gaia Andrea Gozza, Maria Pia Polito, Elena Enzo, Alejandro Sifrim, Mariaceleste Aragona
Lizebona August Ambattu, Blanca del Rosal, Carmelo Ferrai, Leslie Yeo
N2B27 media formulations influence gastruloid development
Tina Balayo, Sharna Lunn, Pau Pascual-Mas, Ulla-Maj Fiuza, Amruta Vasudevan, Joshua D. Frenster, Hannah Y. Galloon, Raquel Flores Peirats, Alfonso Martinez Arias, André Dias, David A. Turner
Alzheimer’s Disease Mutations Disrupt Neural Stem Cell Fate and Early Brain Development
Yiqiao Wang, Johan Lorentz, Elyas Mohammadi, Dilsah Ezgi Yilmaz, Theologos Sgouras, Yuxi Guo, Giusy Pizzirusso, Aphrodite Demetriou, Ivan Nalvarte, Marianne Schultzberg, Xiaofei Li
Manu Araolaza, Iraia Munoa-Hoyos, Itziar Urizar-Arenaza, Irune Calzado, Nerea Subiran
Felicity Ting-Yu Hsu, Rachel Smith-Bolton
Impaired stem cell migration and divisions in Duchenne Muscular Dystrophy revealed by live imaging
Liza Sarde, Gaëlle Letort, Hugo Varet, Vincent Laville, Julien Fernandes, Shahragim Tajbakhsh, Brendan Evano
Epigenetic Reprogramming Alters Intestinal Stem Cell Fate in Pouchitis
Chaoting Zhou, Jing Yu Carolina Cen Feng, Shan Liu, Katherine S. Ventre, Jordan E. Axelrad, Kyung Ku Jang, Ken Cadwell
Alternative splicing dynamics during human cardiac development in vivo and in vitro
Beatriz Gomes-Silva, Marta Furtado, Marta Ribeiro, Sandra Martins, Maria Teresa Carvalho, Henrike Maatz, Michael Radke, Michael Gotthardt, Rosina Savisaar, Maria Carmo-Fonseca
Michelangelo Corcelli, Ellen Petzendorfer, Kate Hawkins, Filipa Vlahova, Catherine Caruso, Mehedi Mohammad Hasan, Katie Durrant, Anna David, Fleur S van Dijk, Pascale V Guillot
Martina Legido, Rebecca Wafer, Marie Srotyr, Parthive H. Patel

Nimmy S. John, Michelle A. Urman, Mahasin G. Mehmood, ChangHwan Lee
Adamo Davide, Genna Vincenzo Giuseppe, Galaverni Giulia, Chiavelli Chiara, Merra Alessia, Lepore Fabio, Boraldi Federica, Quaglino Daniela, Evangelista Jessica, Lococo Filippo, Pellegrini Graziella
Axelle Bouche, Diego Michel, Perrine Castets, Didier Hannouche, Thomas Laumonier
In vivo self-renewal and expansion of quiescent stem cells from a non-human primate
Jengmin Kang, Abhijnya Kanugovi, M. Pilar J. Stella, Zofija Frimand, Jean Farup, Andoni Urtasun, Shixuan Liu, Anne-Sofie Clausen, Heather Ishak, Summer Bui, Soochi Kim, Camille Ezran, Olga Botvinnik, Ermelinda Porpiglia, Mark Krasnow, Antoine de Morree, Thomas A. Rando
Caramai N. Kamei, William G. B. Sampson, Carolin Albertz, Oliver Aries, Amber Wolf, Rohan M. Upadhyay, Samuel M. Hughes, Heiko Schenk, Frederic Bonnet, Bruce W. Draper, Kyle W. McCracken, Denise K. Marciano, Leif Oxburgh, Iain A. Drummond
| Plant development
Ethylene modulates cell wall mechanics for root responses to compaction
Jiao Zhang, Zhuo Qu, Zengyu Liu, Jingbin Li, Edward Farrar, Osvaldo Chara, Lucas Peralta Ogorek, Augusto Borges, Shingo Sakamoto, Nobutaka Mitsuda, Xiaobo Zhu, Mingyuan Zhu, Jin Shi, Wanqi Liang, Malcolm Bennett, Bipin Pandey, Dabing Zhang, Staffan Persson
Arabidopsis PIEZO integrates magnetic field and blue light signaling to regulate root growth
Ziai Peng, Wenjing Yang, Man Dong, Hanrui Bai, Yan Lei, Ninghui Pan, Yong Xie, Liwei Guo, Changning Liu, Yunlong Du
Andrew R.G. Plackett, Marco Catoni

AarMIXTAs: key factors of T-shaped non-glandular trichome development in Artemisia argyi
Xinlian Chen, Duan Wu, Chunyu Li, Baosheng Liao, Qi Shen
Rachel Jouni, Caroline Henry, Sébastien Bélanger, Patricia Baldrich, Blake C. Meyers
The hollow truth: ethylene-triggered ROS, PCD, senescence, and autophagy drive hollow stem formation
Mengxiao Yan, Weijuan Fan, Wei Yang, Jiamin Zhao, Yinghui Meng, Wuyu Zhou, Haiyan Zhuang, Ziyin Xu, Yuqin Wang, Qingjun Huang, Ling Yuan, Hongxia Wang, Jun Yang
Emma White, Matthew J Wilson, Richard Summers, Cristobal Uauy, Andrew J. Fleming
Lipocalins are versatile regulators of development and stress response in mosses
Shuanghua Wang, Jianchao Ma, Qia Wang, Yanlong Guan, Xiangyang Hu, Hang Sun, Jinling Huang
Anna Anielska-Mazur, Julia Rachowka, Lidia Polkowska-Kowalczyk, Michal Krzyszton, Dominika Cieslak, Radoslaw Mazur, Maria Bucholc, Jolanta Rakowska, Dominika Trzmiel, Paulina Stachula, Mateusz Olechowski, Szymon Swiezewski, Grazyna Dobrowolska, Anna Kulik
Marie Alonso, Pierre Prévost, Aline Potier, Pascal GP Martin, Yves Caraglio, Michael Nicolas, Michel Hernould, Christophe Rothan, Béatrice Denoyes, Amèlia Gaston
| Environment, evolution and development
Kazuhide Miyamoto, Junpei Kuroda, Satomi Kamimura, Yasuyuki Sasano, Gembu Abe, Satoshi Ansai, Noriko Funayama, Masahiro Uesaka, Koji Tamura

Wnt signaling restores evolutionary loss of regenerative potential in Hydra
Sergio E Campos, Sahar Naziri, Jackson Crane, Jennifer Tsverov, Ben D Cox, Craig Ciampa, Celina E Juliano
Rapid canalisation of mandible structure in Tetrapoda
Emily Charlotte Watt, Ryan N. Felice, Anjali Goswami
Alexander V. Badyaev, Carmen Sanchez Moreno, Cody A. Lee, Sarah E. Britton, Laurel M. Johnstone, Renee A. Duckworth
Isabella Wilson, Diana Demiyah Mohd Hamdan, Frank Grutzner
The evolution of gene regulation in mammalian cerebellum development
Ioannis Sarropoulos, Mari Sepp, Tetsuya Yamada, Philipp S. L. Schäfer, Nils Trost, Julia Schmidt, Céline Schneider, Charis Drummer, Sophie Mißbach, Ibrahim I. Taskiran, Nikolai Hecker, Carmen Bravo González-Blas, Niklas Kempynck, Robert Frömel, Piyush Joshi, Evgeny Leushkin, Frederik Arnskötter, Kevin Leiss, Konstantin Okonechnikov, Steven Lisgo, Miklós Palkovits, Svante Pääbo, Margarida Cardoso-Moreira, Lena M. Kutscher, Rüdiger Behr, Stefan M. Pfister, Stein Aerts, Henrik Kaessmann
Josane F. Sousa, Gabriela Lima, Louise Perez, Hannah Schof, Igor Schneider
Sharks and rays have the oldest vertebrate sex chromosome with unique sex determination mechanisms
Taiki Niwa, Yoshinobu Uno, Yuta Ohishi, Mitsutaka Kadota, Naotaka Aburatani, Itsuki Kiyatake, Daiki Katooka, Michikazu Yorozu, Nobutaka Tsuzuki, Atsushi Toyoda, Wataru Takagi, Masaru Nakamura, Shigehiro Kuraku
The retinoic acid receptor regulates development of a key evolutionary novelty – the molluscan shell
Keisuke Shimizu, Paul A. O’Neill, Kazuyoshi Endo, Tetsuhiro Kudoh
Gabriele Andreatta, Sara Montagnese, Rodolfo Costa
Jaeyeong Han, Alyson Ficca, Marissa Lanzatella, Kanika Leang, Matthew Barnum, Jonathan C. T. Boudreaux, Nathan E. Schroeder
Dang M. Nguyen, Sarah K. Monroe, Danielle N. Rendina, Kevin S. Boyd, Erika D. Rispoli, Olivia M. Wirfel, A. Brayan Campos-Salazar, Anna R. Araujo, Trisha V. Vaidyanathan, Virginia L. Keziah, Benjamin A. Devlin, Caroline J. Smith, Staci D. Bilbo
Divergence of germ cell-less roles in germ line development across insect species
Jonchee A. Kao, Ben Ewen-Campen, Cassandra G. Extavour
Periklis Paganos, Carsten Wolff, Danila Voronov, S. Zachary Swartz

Cell Biology
Chelsey Lynn Gough, Yuxuan Rain Xiong, Aoi Hiroyasu, Christina Rou Hsu, Viktorija Juciute, MinJee Kim, Kalen Dofher, Kenji Sugioka
N-cadherin facilitates trigeminal sensory neuron outgrowth and target tissue innervation
Caroline A. Halmi, Carrie E. Leonard, Alec T. McIntosh, Lisa A. Taneyhill
Extrinsic Apoptosis and Necroptosis in Telencephalic Development: A Single-Cell Mass Cytometry Study
Jiachen Shi, Weile Liu, Alison Song, Timi Sanni, Amy Van Deusen, Eli R. Zunder, Christopher D. Deppmann
A novel RNP compartment boosts translation in growing mouse oocytes to avoid cytoplasm dilution
N. Zollo, G. Zaffagnini, A. Canette, G. Letort, C. Da Silva, N. Tessandier, J. Dumont, C. Blugeon, S. Lemoine, B. Wattellier, E. Böke, M. Almonacid, M.-H. Verlhac
Nuclear hormone receptor regulation of Caudal mediates ventral nerve cord assembly in C. elegans
Nathaniel Noblett, Tony Roenspies, Stephane Flibotte, Antonio Colavita
Eleanor Martin, Sujasha Ghosh, Yuhong Chen, Rossana Girardello, Barbara Hübner, Kay En Low, Gunnar Dittmar, Alexander Ludwig

Cis-inhibition of Notch by Delta controls follicle formation in Drosophila melanogaster
muriel grammont, Caroline Vachias
Janet Tait, Carmen Marthen, Barbara Hoelscher, Tobias Straub, Ralph A.W. Rupp
Noor M. Kotb, Gulay Ulukaya, Anupriya Ramamoorthy, Lina Seojin Park, Julia Tang, Dan Hasson, Prashanth Rangan
The actin protrusion deforms the nucleus during invasion through basement membrane
Johan d’Humières, Lianzijun Wang, David R. Sherwood, Julie Plastino
Proton-secreting cells modulate mucosal immune surveillance in the male reproductive tract
AAS Da Silva, F Barrachina, MC Avenatti, ML Elizagaray, I Bastepe, E Sasso-Cerri, MA Battistone
Modelling
Tissue Fluidity: A Double-Edged Sword for Multicellular Patterning
Rikki M. Garner, Sean E. McGeary, Allon M. Klein, Sean G. Megason
S Ben Tahar, E Comellas, TJ Duerr, JR Monaghan, JJ Munoz, SJ Shefelbine
Stem-cell differentiation underpins reproducible morphogenesis
Dominic K Devlin, Austen RD Ganley, Nobuto Takeuchi
A model for boundary-driven tissue morphogenesis
Daniel S. Alber, Shiheng Zhao, Alexandre O. Jacinto, Eric F. Wieschaus, Stanislav Y. Shvartsman, Pierre A. Haas
Tools & Resources
A human induced pluripotent stem cell toolbox for studying sex chromosome effects
Ruta Meleckyte, Wazeer Varsally, Jasmin Zohren, Jerry Eriksson, Tania Incitti, Linda Starnes, Amy Pointon, Ryan Hicks, Benjamin E. Powell, James M.A. Turner
Genetic context of transgene insertion can influence neurodevelopment in zebrafish
Anna J. Moyer, Alexia Barcus, Mary E.S. Capps, Jessica A. Chrabasz, Robert L. Lalonde, Christian Mosimann, Summer B. Thyme
Melissa Antoniou-Kourounioti, Felícitas Ramírez de Acuña, Raphael Caspar Constantin Schettler, Sakshi Kaushal Udar, Aitor Hamzic Petite, Ramayee Vidhya Sivasubramanian, Andrea Erika Münsterberg, Timothy Grocott
Step-by-step protocol for making a knock-in Xenopus laevis to visualize endogenous gene expression
Norie Kagawa, Yoshihiko Umesono, Ken-Ichi T Suzuki, Makoto Mochii
Bernadette Banrezes, Thierry Sainte Beuve, Anne Frambourg, Alice Jouneau
Multi-omic human neural organoid cell atlas of the posterior brain
Nadezhda Azbukina, Zhisong He, Hsiu-Chuan Lin, Malgorzata Santel, Bijan Kashanian, Ashley Maynard, Tivadar Török, Ryoko Okamoto, Marina Nikolova, Sabina Kanton, Valentin Brösamle, Rene Holtackers, J. Gray Camp, Barbara Treutlein
In vitro approaches to study centriole and cilium function in early mouse embryogenesis
Isabella Voelkl, Tamara Civetta, Mirijam Egg, Marie Huber, Songjie Feng, Alexander Dammermann, Christa Buecker
Yolanda W. Chang, Marjolein Trimp, Talia Van Der Helm, Albert Blanch-Asensio, Arend W. Overeem, Susana M. Chuva De Sousa Lopes
Enhancing Embryo Stage Classification with Multi-Focal Plane Imaging and Deep Learning
Aissa Benfettoume Souda, Wistan Marchadour, Souaad Hamza-Cherif, Jean-Marie Guyader, Marwa Elbouz, Fréderic Morel, Aurore Perrin, Mohammed El Amine Bechar, Nesma Settouti

Automated, high-throughput in-situ hybridization of Lytechinus pictus embryos
Yoon Lee, Chloe Jenniches, Rachel Metry, Gloria Renaudin, Svenja Kling, Evan Tjeerdema, Elliot W Jackson, Amro Hamdoun
Wietske A.P. Bastiaansen, Melek Rousian, Anton H.J. Koning, Wiro J. Niessen, Bernadette S. de Bakker, Régine P.M. Steegers-Theunissen, Stefan Klein
Research practice & education
Daniel A. Gorelick, Alejandra Clark
LGBTQ+ realities in the biological sciences
Katelyn M. Cooper, Carly A. Busch, Alice Accorsi, Derek A. Applewhite, Parth B. Bhanderi, Bruno da Rocha-Azevedo, Abhijit Deb Roy, Joseph P. Campanale, Fred Chang, Jerry E. Chipuk, Lee A. Ligon, G.W. Gant Luxton, Austin J. Graham, Camila Hochman-Mendez, Imge Ozugergin, Zachory M. Park, Claire M. Thomas, Alex M. Valm, Hongxian Zhu, Rebecca S. Alvania
Cracking the code of co-authorship networks geo-temporally using interpretable machine learning
Swapnil Keshari, Zarifeh Heidari Rarani, Akash Kishore, Jishnu Das