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Dynamic display of data with animated plots

Posted by , on 27 April 2020

Data from time-lapse experiments is often displayed in a graph or plot, to visualize the dynamics of biological systems (Goedhart, 2020). Ironically, the perception of the dynamics is largely lost ...
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Data manipulation? It's normal(ization)!

Posted by , on 25 June 2019

In a previous blog, I have highlighted several ways to visualize the cell-to-cell heterogeneity from time-lapse imaging data. However, I have ignored that data is often rescaled in a way ...
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Visualizing the heterogeneity of single cell data from time-lapse imaging

Posted by , on 12 December 2018

When we examined the kinetics of Rho GTPase activity in endothelial cells in response to receptor stimulation (Reinhard, 2017), we noticed considerable cell-to-cell heterogeneity. In the original work we published ...
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Make a difference: the alternative for p-values

Posted by , on 8 October 2018

Calculation and reporting of p-values is common in scientific publications and presentations (Cristea and Ioannidis, 2018). Usually, the p-value is calculated to decide whether two conditions, e.g. control and treatment, ...
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Visualizing data with R/ggplot2 - One more time

Posted by , on 26 June 2018

Experiments are rarely performed in isolation. Usually, several conditions are compared in parallel or sequential experiments. This experimental strategy also applies to time-dependent data, e.g. from timelapse imaging. So, naturally, ...
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Visualizing data with R/ggplot2 - It’s about time

Posted by , on 31 May 2018

The visualization of temporal data by line graphs has been documented and popularized by William Playfair in the 18th century (Aigner et al, 2011; Beniger and Robyn, 1978). Today, time-dependent changes ...
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Converting excellent spreadsheets to tidy data

Posted by , on 6 October 2017

Structuring data according to the ‘tidy data‘ standard simplifies data analysis and data visualisation. But understanding the structure of tidy data does not come naturally (in my experience), since it ...
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