Seema Grewal, Author at the Node

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 141, Issue 2)

Posted by Seema Grewal, on 31 December 2013

Here are the highlights from the current issue of Development: Peri important role for Notch Pericytes are specialised cells that wrap around the endothelial cells of the vasculature to ...

In Development this week (Vol. 141, Issue 1)

Posted by Seema Grewal, on 17 December 2013

Here are the highlights from the current issue of Development: GABAB inhibits neural stem cell proliferation Neurotransmitter functions are typically associated with neural function rather than with development, but ...

In Development this week (Vol. 140, Issue 24)

Posted by Seema Grewal, on 3 December 2013

Here are the highlights from the current issue of Development: One-step transdifferentiation Terminally differentiated cells are generally considered to be in a developmentally locked state in vivo; they are ...

In Development this week (Vol. 140, Issue 23)

Posted by Seema Grewal, on 19 November 2013

Here are the highlights from the new issue of Development: Broken-hearted over Hippo Mammalian cardiac regeneration is greatly impeded by the massive loss of cardiomyocytes that occurs following acute ...

In Development this week (Vol.140, Issue 22)

Posted by Seema Grewal, on 5 November 2013

Here are the highlights from the current issue of Development: Profiling the mammalian brain In mammals, adult neurogenesis is highly restricted to the subventricular zone and to the subgranular ...

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps

by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010