Seema Grewal, Author at the Node

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 141, Issue 7)

Posted by Seema Grewal, on 18 March 2014

Here are the highlights from the current issue of Development: A vascular scaffold for islet nerve growth In many organs, the processes of vascularisation and innervation are frequently interdependent. ...

In Development this week (Vol. 141, Issue 6)

Posted by Seema Grewal, on 4 March 2014

Here are the highlights of the current issue of Development: Auxin biosynthesis: the root of xylem patterning In the Arabidopsis root, xylem is organised into a central file of metaxylem that is ...

In Development this week (Vol. 141, Issue 5):

Posted by Seema Grewal, on 18 February 2014

Here are the highlights from the current issue of Development: Time to update the mammalian CV The dorsal aorta (DA) and the cardinal vein (CV) are the first vascular ...

In Development this week (Vol. 141, Issue 4)

Posted by Seema Grewal, on 4 February 2014

Here are the highlights from the current issue of Development: Integrating integrin signals for neocortical growth Within the developing neocortex, multiple progenitor cell types contribute to neuronal production, and ...

In Development this week (Vol. 141, Issue 3)

Posted by Seema Grewal, on 21 January 2014

Here are the highlights from the current issue of Development: Hoxb1b gets the neural tube into shape Hox genes are classically known for their roles in patterning the anterior-posterior ...

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps

by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010