Seema Grewal, Author at the Node

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 144, Issue 19)

Posted by Seema Grewal, on 3 October 2017

Here are the highlights from the current issue of Development: Making a move: EMT holds the key to planarian regeneration During development and wound healing, progenitor cells are required ...

In Development this week (Vol. 144, Issue 18)

Posted by Seema Grewal, on 19 September 2017

Here are the highlights from the current issue of Development: Separating nematode species by spermatogenesis During spermatogenesis, unnecessary organelles and cytoplasmic components are shed from developing sperm in order ...

In Development this week (Vol. 144, Issue 14)

Posted by Seema Grewal, on 18 July 2017

Here are the highlights from the current issue of Development: Computing branching pattern complexity Bile – a fluid that aids digestion – is transported from the liver to the ...

In Development this Week (Vol. 144, Issue 13)

Posted by Seema Grewal, on 4 July 2017

Here are the highlights from the current issue of Development: Heart tube formation: a gut reaction Morphogenesis of the endoderm-derived foregut (FG) is tightly linked to that of the ...

In Development this week (Vol. 144, Issue 9)

Posted by Seema Grewal, on 2 May 2017

Here are the highlights from the current issue of Development: SEGGA: new software for analysing cell shape and dynamics Changes in epithelial cell shape and organisation are essential for ...

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps

by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010