Seema Grewal, Author at the Node

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 144, Issue 8)

Posted by Seema Grewal, on 11 April 2017

Here are the highlights from the current issue of Development: A new niche for human HSCs Human haematopoiesis occurs at various anatomical sites throughout development, including the yolk sac, ...

In Development this week (Vol. 144, Issue 6) - SPECIAL ISSUE ON ORGANOIDS

Posted by Seema Grewal, on 14 March 2017

The current issue of Development – our Special Issue on Organoids – features a collection of review- and research-based articles focusing on organoids. Here are some of the highlights. Happy ...

In Development this week (Vol. 144, Issue 5)

Posted by Seema Grewal, on 28 February 2017

Here are the highlights from the new issue of Development: Adding a new layer of complexity to pre-eclampsia Pre-eclampsia (PE) is a pregnancy complication associated with abnormal formation of ...

In Development this week (Vol. 144, Issue 4)

Posted by Seema Grewal, on 14 February 2017

Here are the highlights from the new issue of Development: A new mechanism in ESC lineage priming Histone demethylases have recognized roles in the control of gene expression during ...

In Development this week (Vol. 144, Issue 3)

Posted by Seema Grewal, on 31 January 2017

Here are the highlights from the current issue of Development: Evolving an atypical developmental programme with IMM The brown alga Ectocarpus has alternating haploid (gametophyte) and diploid (sporophyte) generations. ...

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps

by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010