Seema Grewal, Author at the Node

Seema Grewal

Posts by Seema Grewal

In Development this week (Volume 137, Issue 16)

Posted by Seema Grewal, on 27 July 2010

Here are the highlights from this week’s issue of Development: Lymphatic networks follow arterial lead The vertebrate lymphatic system consists of lymphatic vessels, which collect fluid from the tissues and return it ...

In Development this week (Volume 137, Issue 15)

Posted by Seema Grewal, on 13 July 2010

Here are the research highlights from the new issue of Development… TORc1-ing about stem cell differentiation In adult tissues, the tight regulation of stem cell selfrenewal and differentiation maintains tissue ...

In Development, Vol 137 (Issue 14)

Posted by Seema Grewal, on 23 June 2010

Here are the research highlights from the current issue of Development. You can find these on the Development site but we thought it would be useful to have them posted ...

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps

by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010

Seema Grewal

Posts by Seema Grewal

In Development this week (Volume 137, Issue 16)

In Development this week (Volume 137, Issue 15)

In Development, Vol 137 (Issue 14)

Recent comments by Seema Grewal