The community site for and by
developmental and stem cell biologists

Seema Grewal

Posts by Seema Grewal

In Development This Week (Vol. 138, Issue 22)

Posted by , on 25 October 2011

Here are the highlights from the current issue of Development: The skin-healing touch of Lhx2 Skin repair after injury involves the recruitment of undifferentiated progenitor cells from nearby hair follicles ...

In Development this week (Vol. 138, Issue 21)

Posted by , on 11 October 2011

Here are the highlights from the current issue of Development: New blood: vasculature restrains pancreas growth Although the primary function of blood vessels is to provide organs with the oxygen ...

In Development this week (Vol. 138, Issue 20)

Posted by , on 22 September 2011

Here are the highlights from the current issue of Development: ApoE: a role in neurogenesis Hippocampal neurodegeneration occurs in most forms of dementia, including Alzheimer’s disease. There is, therefore, intense ...

In Development this week (Vol. 138, Issue 19)

Posted by , on 6 September 2011

Here are the research highlights form the current issue of Development: Modelling liver development with ES cells: HNF4A is key Human embryonic stem cells (hESCs), via their ability to differentiate ...

In Development this week (Vol. 138, Issue 18)

Posted by , on 23 August 2011

Here are the research highlights from the current issue of Development: Shaping up the Hippo pathway The Hippo pathway, which regulates cell proliferation, is regulated by cell density: low cell ...

Recent comments by Seema Grewal

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps
by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010