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developmental and stem cell biologists

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 138, Issue 17)

Posted by , on 9 August 2011

Here are the highlights from the current issue of Development: Human embryos make an early transcriptional start Human preimplantation development is a highly dynamic process that lasts about 6 days. ...

In Development this week (Vol. 138, Issue 16)

Posted by , on 26 July 2011

Here are the research highlights from the current issue of Development: Pushing the nuclear envelope Not all nuclei are regular spheres as is often shown in textbooks. For example, in ...

In Development this week (Vol. 138, Issue 15)

Posted by , on 12 July 2011

Here are the research highlights from the current issue of Development: BMP signalling rolls up in the neural tube During neurulation, polarised cell-shape changes at hinge points – specialised regions ...

In Development this week (Vol. 138, Issue 14)

Posted by , on 21 June 2011

Here are the research highlights from the current issue of Development: How to make stripes: revising the pair-rules A key step in Drosophila segmentation is the transition from non-periodic to ...

In Development this week (Vol. 138, Issue 13)

Posted by , on 7 June 2011

Here are the highlights from the current issue of Development: Mutant Xist merely muffles X chromosome In XX female mammals, inactivation of one X chromosome during development equalises the levels ...

Recent comments by Seema Grewal

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps
by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010