Seema Grewal, Author at the Node

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 138, Issue 17)

Posted by Seema Grewal, on 9 August 2011

Here are the highlights from the current issue of Development: Human embryos make an early transcriptional start Human preimplantation development is a highly dynamic process that lasts about 6 days. ...

In Development this week (Vol. 138, Issue 16)

Posted by Seema Grewal, on 26 July 2011

Here are the research highlights from the current issue of Development: Pushing the nuclear envelope Not all nuclei are regular spheres as is often shown in textbooks. For example, in ...

In Development this week (Vol. 138, Issue 15)

Posted by Seema Grewal, on 12 July 2011

Here are the research highlights from the current issue of Development: BMP signalling rolls up in the neural tube During neurulation, polarised cell-shape changes at hinge points – specialised regions ...

In Development this week (Vol. 138, Issue 14)

Posted by Seema Grewal, on 21 June 2011

Here are the research highlights from the current issue of Development: How to make stripes: revising the pair-rules A key step in Drosophila segmentation is the transition from non-periodic to ...

In Development this week (Vol. 138, Issue 13)

Posted by Seema Grewal, on 7 June 2011

Here are the highlights from the current issue of Development: Mutant Xist merely muffles X chromosome In XX female mammals, inactivation of one X chromosome during development equalises the levels ...

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps

by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010