Closing Date: 18 April 2021
We offer one fully funded postdoctoral position up to five years in the Laboratory of Genome Integrity located at the National Institutes of Health (NIH, Bethesda, MD). NIH is the largest biomedical research agency in the world, fosters world-renowned researchers and provides access to state-of-the art innovative technologies and scientific resources.
Our laboratory uses human and mouse embryonic stem cells (ESCs) as well as mouse embryos to understand the molecular mechanisms underlying cell fate decisions. The applicant should have or about to have a PhD in Developmental Biology, Genetics or similar, and must have demonstrated expertise on molecular biology/mammalian cell culture (preferably in embryonic stem cells). Knowledge in mouse embryology, single-cell RNAseq, chromatin architecture and/or next generation sequencing technologies will be considered as an advantage.
The applicant will be involved in a very exciting project investigating the relation between cell plasticity/totipotency and chromatin architecture (see our last publication about this topic, https://doi.org/10.1101/2020.12.20.423692). We seek a highly motivated, creative individual, eager to learn and develop new technologies and complex cell systems based on live cell/embryo imaging, single-cell technologies and CRISPR-based editing interested in understanding how a single cell can develop into a complex multicellular organism in vitro and in vivo.
Please send a brief cover letter, CV and three reference letters via e-mail to:
- Vega-Sendino, et al (2021) The ETS Transcription Factor ERF controls the exit from the naïve pluripotent state. BioRxiv, doi: https://doi.org/10.1101/2021.02.01.429223.
- Olbrich, T., et al (2020) CTCF is a barrier for totipotent-like reprogramming. BioRxiv, doi: https://doi.org/10.1101/2020.12.20.423692.
- Markiewicz-Potoczny, M., et al (2020) TRF2-independent protection of telomeres in pluripotent stem cells. Nature, 589: 103-109.
- Mayor-Ruiz C, et al. ERF deletion rescues RAS deficiency in mouse embryonic stem cells. Genes & Dev. 32: 568-576, 2018.
- Ruiz S, et al. Limiting replication stress during somatic cell reprogramming reduces genomic instability in induced pluripotent stem cells. Nature Commun. 6: 8036, 2015.