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Displaying posts with the tag: genome-editing [Clear Filter]

Laboratory Research Scientist

Posted by on September 20th, 2017

OVERVIEW   We are seeking a highly motivated and collaborative Laboratory Research Scientist in the area of human embryology and stem cell biology to join Dr. Kathy Niakan’s laboratory. The lab has identified several signalling pathways that may be operational in the human embryo to regulate the establishment or maintenance of pluripotent epiblast progenitor cells[…]

Sweetening with a pinch of salt: maximized Cas9 efficiency in zebrafish

Posted by on June 14th, 2016

  Alexa Burger, Mosimann lab, Institute of Molecular Life Sciences, University of Zürich, Switzerland. When I first heard about the “new” genome editing method in early 2013 called CRISPR-Cas9, I thought: “Never ever again will I work with targeted nucleases!” Now it’s mid-2016, we published our approaches to maximize Cas9 effectiveness in zebrafish with Development[…]

Meeting Report: Genome Editors and CRISPRs meeting, Edinburgh 23rd April 2015

Posted by on May 5th, 2015

Around 200 scientists from several Edinburgh research institutes gathered in central Edinburgh to discuss the current state and uses of zinc finger nucleases, TALENs (TAL effector nucleases) and CRISPR/Cas9 technology. The one-day meeting covered applications ranging from creating transgenic zebrafish lines to carrying out disease-relevant genetic modification of human cells. The meeting was lively and[…]

Postdoctoral scientist position in Genome Editing in Xenopus at the Marine Biological Laboratory

Posted by on February 25th, 2015

A Postdoctoral Scientist position is available in the Horb Laboratory of the Eugene Bell Center for Regenerative Biology & Tissue Engineering at the Marine Biological Laboratory (http://www.mbl.edu/horb/). The position will focus on the creation of Xenopus models of human disease through the generation of mutants using CRISPR/Cas and TALENs and to work on developing and[…]

E-CRISP: Design of custom gRNA constructs

Posted by on August 30th, 2013

E-CRISP is now available under: www.e-crisp.org Genome editing by applying the CRISPR/Cas system has been shown to be a promising new tool in genetics. CRISPR/Cas works by guided DNA double strand breaks (DSB) at specific loci in the genomic or exogenic DNA, where various kinds of sequence alterations can be introduced (exploiting the cellular DSB-repair[…]

Zinc Finger Nucleases targeting genes in a frog near you!

Posted by on May 9th, 2011

Loss-of-function studies in Xenopus have been, until recently, limited to transient knockdowns by injection of morpholino antisense oligonucleotides.  In part because of X. laevis’ complex allotetraploid genome, the system lacked techniques for targeted gene disruption.  In recent years the use of the closely related Xenopus tropicalis, a true diploid with one of the smallest tetrapod[…]