Lab meeting with the Harnoš lab

Where is the lab?

The lab is located in Brno, Czech Republic, on the modern campus. 

Lab website: https://www.sci.muni.cz/ofiz/en/harnos-lab-2/

Research summary

Jakub ‘James’ Harnos: Polarity refers to spatial differences in shape, structure, and function within a cell. Almost all cell types exhibit some form of polarity that enables them to carry out specialized functions. We focus on planar polarity, which refers to the coordinated alignment of cells across the tissue plane. Planar polarity is currently viewed as a “passive” compass providing cells with a feel of direction.

Our first aim is to show the active role of planar polarity in neural tube formation. Neural tube formation is an early developmental event that comprises the actions of approximately two hundred proteins. Despite the tube formation is described somewhat well, knowledge of what triggers its initiation is lacking. We have collected evidence that polarity proteins may be the missing active factors for initiating neural tube formation.

Our second aim deals with cell migration. Migration is the directed movement of a cell from one place to another and requires an increased amount of energy. The produced energy is used for the cytoskeletal rearrangement of dedicated regions in a migratory cell, thus allowing its physical movement. However, it remains unknown which signal actively instructs cells to produce more energy needed for rearrangements. Here, we aim to show the active role of planar polarity as an energy trigger for cell migration.

In sum, assigning dynamic behaviors to polarity proteins is what defines the Harnos lab.

Lab roll call

Katarzyna Anna Radaszkiewicz, PhD, MSc, a Polish postdoc, investigates cell migration, tissue culture, and the cytoskeleton, with a focus on the position of mitochondria during cell migration.

Lorena Agostini Maia, PhD, MSc, a Brazilian postdoc, studies neurulation in Xenopus and the WNT pathway in development.

Petra Paclikova, a Czech postdoc, specializes in WNT signaling and conducts metabolic assays.

Nela Leksova, a Slovak undergraduate student, works on neurulation in Xenopus.

Bc. Aneta Poukova, a Czech undergraduate student, examines the interaction between PCP and mitochondrial proteins, while Bc. Pavla Kolarova, also a Czech undergraduate student, contributes to the lab though her specific research focus is not detailed.

Sarka Novotna, MSc, a Czech PhD student, develops optogenetic tools in Xenopus, bringing expertise in biophysics and microscopy.

Marek Dokoupil, a Czech undergraduate student, explores the crosstalk between Wnt and Notch signaling pathways, a topic also researched by Bc. Hana Suchankova and Bc. Kristyna Daniela Krutova, both Czech undergraduate students.

Julie Netusilova, MSc, a Czech lab technician, manages the lab and takes care of the frogs, alongside MV. Douglas Porto, a Brazilian veterinarian responsible for the care of the frogs.

Favourite technique, and why?

James: My favorite technique is microscopy because of its remarkable ability to provide detailed and insightful imaging at the cellular and molecular levels. The precision and clarity it offer are essential for exploring and understanding complex biological processes.

Apart from your own research, what are you most excited about in developmental and stem cell biology?

James: I’m excited about the interplay of signaling pathways and advancements in organoid technology, which offer great potential for breakthroughs in developmental and stem cell biology.

How do you approach managing your group and all the different tasks required in your job?

James: I manage my group by setting clear goals, prioritizing tasks, and maintaining open communication. I also use planning tools to keep track of progress and ensure that everyone stays on track.

To all lab members: What is the best thing about where you work? 

The best thing about where I work is the collaborative and supportive atmosphere. I like the modern approach, the desire to move on and discover new things. I really enjoy working with our model organisms and being able to try new lab procedures.

The best thing about where I work is the quality of our academic environment, which seamlessly combines warmth, productivity, scientific and technical excellence, and comprehensive support. It’s a welcoming space that fosters respect, empathy, and collaboration, ensuring that all feel valued and supported.

One of the best things about where I work is the familiar atmosphere. We have a collaborative environment where everybody is so nice and respectful. The lab, as well as the department, is very friendly and welcoming, making it a truly enjoyable place to be every day and, besides, doing what I love: being a frog researcher.

Friendly team.  Everyone in the lab is always nice, I can ask anyone for help and I’m not afraid to admit when I screw something up. Of course, I also enjoy the work I do in the lab. I really like working with frogs, but it’s great that I have the opportunity to try other techniques.

There is a friendly atmosphere, we also have access to a microscopy facility, which is located on the campus. Besides that, we have modern equipment in our laboratories.

I like our campus, because it is new and we have modern equipment. Everybody in the lab is always very helpful and there is a nice and friendly environment.

The highlight of working in our research lab is the positive environment that encourages communication and collaboration both within our team and with other groups. This supportive atmosphere not only helps us tackle research challenges but also supports mental well-being. Additionally, Jakub’s enthusiasm and energy drive projects forward, creating a dynamic and inspiring research environment.

Acceptance and understanding within the lab members. Professional approach and mentoring. I appreciate the will to find the best compromise between both my needs and Jakub’s vision. 

My coworkers. They are all friendly and kind. Whenever I need advice, there will always be someone who is willing to help me.

To all lab members: What’s there to do outside of the lab?

I appreciate that our team spends time together outside the lab. Besides attending school events, we also enjoy meeting up at the pub for some friendly gatherings.

Outside of the lab, Brno offers a wealth of activities with its rich cultural, social, and natural attractions. You can visit renowned theaters, history-rich museums, and vibrant local arts.

The city provides a welcoming and inclusive atmosphere, perfect for socializing and community events. Brno’s natural beauty is highlighted by well-maintained parks, serene nature reserves, and ample green spaces for relaxation and recreation. Efficient public transportation makes it easy to explore all these offerings.

Brno is a university town and, for a small city, it is packed full of great things to see and do, bringing rich cultural and social life all year round. With a friendly atmosphere, Brno is also great for outdoor activities. A short ride on the bus or train, or even a walk, can take you to a calm place surrounded by nature.

I appreciate that next to the campus is a shopping center, so I can buy almost everything I need here. In the city, there are many swimming pools, gyms, and beautiful Christmas markets in the winter.

Brno is a great city to live in. There are nice parks, museums, concerts and many pubs!

Brno is perfectly situated for quick escapes into nature. With numerous opportunities for hiking, biking, climbing, and wildlife watching, it is an ideal destination for both casual nature lovers and outdoor enthusiasts. The city has plenty to offer for those who love urban living.

As a student city, it features numerous spots where you can enjoy a pleasant evening and drink tasty Czech beer.

Lots of things, including enjoying Brno’s gastronomy, music concerts and outside cinemas in summer. At-home-activities such as art, sewing, crocheting and playing videogames with my boyfriend.

There are many possibilities; however, our most popular activity is going to a restaurant for a dinner and beer.

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