Seema Grewal, Author at the Node

Seema Grewal

Posts by Seema Grewal

An Introduction to Writing Review Articles

Posted by Seema Grewal, on 7 April 2020

Last week, I gave a talk (online, of course) about ‘Writing review articles’. It was aimed at graduate students who, as part of their training, had to identify a topic ...

In Development this week (Vol 145, Issue 1)

Posted by Seema Grewal, on 16 January 2018

The first Development issue of the year is now complete! Here are the highlights: RESEARCH HIGHLIGHTS: Micro-lenses focus on cataract development Cataracts have many potential risk factors but ...

In Development this week (Vol. 144, Issue 24)

Posted by Seema Grewal, on 18 December 2017

Here are the highlights from the current issue of Development – the last one of the year! Happy reading…and happy holidays! Branched actin keeps Nrf2 in check in the ...

In Development this week (Vol. 144, Issue 22)

Posted by Seema Grewal, on 14 November 2017

Here are the highlights form the current issue of Development: Cross-border control of stem cell behaviour Cell identity and proliferation differs between organs, raising the question of how cells ...

In Development this week (Vol. 144, Issue 21)

Posted by Seema Grewal, on 31 October 2017

Here are the highlights form the current issue of Development: Making matrix in the inner ear The tectorial membrane (TM) is an extracellular matrix (ECM) that overlies the organ ...

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps

by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010