Seema Grewal, Author at the Node

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 144, Issue 2)

Posted by Seema Grewal, on 17 January 2017

Here are the highlights from the current issue of Development: ECM: bridging the gap between germ layers The extracellular matrix (ECM) plays crucial roles during morphogenesis but how it ...

In Development this week (Vol. 144, Issue 1)

Posted by Seema Grewal, on 3 January 2017

Here are the highlights from the new issue of Development – the first one of the year. Happy reading…and Happy New Year! Embryos rewired: the changing metabolome of early ...

In Development this week (Vol. 143, Issue 24)

Posted by Seema Grewal, on 13 December 2016

Here are the highlights from the new issue of Development…the last one of the year! SETting chromatin state through transcription Setd5 is a poorly characterised murine member of the ...

In Development this week (Vol. 143, Issue 23)

Posted by Seema Grewal, on 29 November 2016

Here are the highlights from the current issue of Development: Syndecan 4 lets lymphatic endothelial cells go with the flow Fluid flow is known to play a role in ...

In Development this week (Vol. 143, Issue 21)

Posted by Seema Grewal, on 1 November 2016

Here are the highlights from the current issue of Development: Signalling cross-talk in the plant root In plants, root architecture is responsive to environmental changes. The plant hormones cytokinin, ...

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps

by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010