Seema Grewal, Author at the Node

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 143, Issue 20)

Posted by Seema Grewal, on 18 October 2016

Here are the highlights from the new issue of Development: Glucocorticoid and STAT3: tipping the balance in the lung The epithelial cells found at the distal tips of the ...

In Development this week (Vol. 143, Issue 19)

Posted by Seema Grewal, on 4 October 2016

Here are the highlights from the current issue of Development: Defining digit number in the limb During vertebrate limb development, multiple mechanisms act to ensure the appropriate number, identity ...

In Development this week - Special Issue on Plant Development

Posted by Seema Grewal, on 13 September 2016

The current issue of Development – our ‘Special Issue on Plant Development’ – contains a collection of review- and research-based articles focusing on plant development. Below, you can find details ...

In Development this week (Vol. 143, Issue 17)

Posted by Seema Grewal, on 30 August 2016

Here are the highlights from the current issue of Development: Coordinating neuronal specification and differentiation Neurogenesis – the process of making new neurons – is indispensable for normal development ...

In Development this week (Vol. 143, Issue 15)

Posted by Seema Grewal, on 2 August 2016

Here are the highlights from the current issue of Development: Defining Polycomb complexes with AEBP2 Polycomb repressive complex 2 (PRC2) directs methylation of histone H3 K27 (H3K27me), a repressive ...

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps

by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010