Seema Grewal, Author at the Node

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 143, Issue 14)

Posted by Seema Grewal, on 19 July 2016

Here are the highlights from the current issue of Development: Gestational stress: at the heart of birth defects Congenital heart disease (CHD) is the most common form of human ...

In Development this week (Vol. 143, Issue 13)

Posted by Seema Grewal, on 5 July 2016

Here are the highlights from the current issue of Development: A pioneer role for PBX1 in neurogenesis The adult rodent subventricular zone (SVZ) is a neurogenic niche that provides ...

In Development this week (Vol. 143, Issue 12)

Posted by Seema Grewal, on 14 June 2016

Here are the highlights from the current issue of Development: A distinct cartilage programme for bone regeneration Bone healing, for example fracture repair in humans, often involves a cartilage ...

In Development this week (Vol. 143, Issue 11)

Posted by Seema Grewal, on 31 May 2016

Here are the highlights from the current issue of Development: A new paradigm for Wnt/β-catenin signalling The Wnt/β-catenin signalling pathway is a key pathway involved in a myriad of ...

In Development this week (Vol. 143, Issue 10)

Posted by Seema Grewal, on 17 May 2016

Here are the highlights from the current issue of Development: How the zebrafish got its tail Posterior axis elongation is a crucial process during metazoan development. In principle, axial ...

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps

by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010