Seema Grewal, Author at the Node

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 143, Issue 9)

Posted by Seema Grewal, on 3 May 2016

Here are the highlights from the current issue of Development: Making inroads into spermatogonial differentiation Differentiation of spermatogonial cells is a crucial part of spermatogenesis. Many of the key ...

In Development this week (Vol. 143, Issue 8)

Posted by Seema Grewal, on 19 April 2016

Here are the highlights from the current issue of Development: The origins of lung macrophages revealed Tissue-resident macrophages are phagocytic cells that are essential for the response to injury ...

In Development this week (Vol. 143, Issue 7)

Posted by Seema Grewal, on 5 April 2016

Here are the highlights from the current issue of Development: TUBB5 analysis yields insights into microcephaly Mutations affecting tubulin genes have been implicated in a range of human neurological ...

In Development this week (Vol. 143, Issue 6)

Posted by Seema Grewal, on 15 March 2016

Here are the highlights from the current issue of Development: Knocking the SOX off obesity Growth restriction in utero is associated with increased risk of obesity in later life. ...

In Development this week (Vol. 143, Issue 5)

Posted by Seema Grewal, on 1 March 2016

Here are the highlights from the current issue of Development: Mak(or)in’ the switch to adulthood The juvenile-to-adult (J/A) transition of many animals, from worms to humans, is regulated by ...

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps

by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010