Seema Grewal, Author at the Node

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 143, Issue 4)

Posted by Seema Grewal, on 16 February 2016

Here are the highlights from the current issue of Development: Fishing out a new role for endocannabinoids Endocannabinoids (ECs) are signalling molecules that regulate appetite, mood and pain, and ...

In Development this week (Vol. 143, Issue 3 )

Posted by Seema Grewal, on 2 February 2016

Here are the highlights from the current issue of Development: New insights into human neural crest induction Human neural crest (NC) cells are the precursors for a wide range ...

In Development this week (Vol. 143, Issue 2)

Posted by Seema Grewal, on 20 January 2016

Here are the highlights from the current issue of Development: Fishing out a role for Caveolin 1 in heart regeneration Unlike the adult mammalian heart, the adult zebrafish heart ...

In Development this week (Vol. 143, Issue 1)

Posted by Seema Grewal, on 5 January 2016

Here are the highlights from the current issue of Development: Making and shaping the lung epithelium Gas exchange in the lung occurs across the alveolar epithelium, which consists of ...

In Development this week (Vol. 142, Issue 24)

Posted by Seema Grewal, on 15 December 2015

Here are the highlights from the current issue of Development: New markers for human endoderm differentiation The generation of mature cell types from pluripotent stem cells (PSCs) relies on ...

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps

by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010