Associate Reviews Editor (Stem Cells) Development
Based in Cambridge, UK

Applications are sought for a new role of Associate Reviews Editor for the stem cell field at Development.

Joining an experienced, expanding and successful team, this is an exciting opportunity for a promising scientific editor to make a significant contribution to one of the major journals in the field of developmental biology. Development publishes primary research articles, reviews and other front section content. The journal is expanding its focus in the stem cell field, and is seeking to appoint a specialist editor to cover this growing area.

Applicants will hold a PhD, ideally in stem cell biology, although outstanding candidates with experience in other areas of developmental biology and with a strong interest in the stem cell field will be considered. Post-doctoral and/or previous editorial experience are highly desirable, although we will provide on-the-job training. The successful candidate will have a broad interest in science, the scientific community and publishing. Excellent interpersonal and literary skills, enthusiasm and commitment are also essential requirements for the position.

Core responsibilities:
• Commissioning, handling peer review and developmental editing of material for the front section of the journal
• Writing press releases, article highlights and material for Development’s community website ‘the Node’
• Representation of the journal at international conferences and within the wider scientific community
• Creative involvement in the journal’s development

The new Associate Reviews Editor will work alongside an experienced in-house team, including the Executive Editor and current Reviews Editor, as well as with our international team of academic editors. Additional responsibilities may be provided for the right candidate.

The position represents a unique opportunity to gain experience on a highly successful life-science journal and offers an attractive salary and benefits. The position will be based in our office in Cambridge, and the contract will be for a three-year period.

The Company of Biologists (www.biologists.com) is a not-for-profit organisation, publishing five journals in the biological sciences: the three established journals Development, Journal of Cell Science and The Journal of Experimental Biology, as well as two newer Open Access journals, Disease Models & Mechanisms and Biology Open. The organisation has an active programme of charitable giving for the further advancement of biological research, including travelling fellowships for junior scientists and contributions to academic societies and conferences.

Applicants should send a CV along with a covering letter that summarises their relevant experience and why they are enthusiastic about this opportunity, and states their salary expectations.

Applications should be sent by email no later than November 26th to miriam@thecob.co.uk
Informal queries to Miriam Ganczkowski on +44 (0)1223 426 164

Applicants must be eligible to work in the UK.

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Here are the highlights from the current issue of Development:

Dishevelled: the Notch-Wnt go-between

The Notch and Wnt signalling pathways are used during animal development to generate a diverse array of cell types. The two pathways often have opposing effects on cell-fate decisions but some cells receive inputs from both pathways simultaneously. In these circumstances, it is common for the receiving cell to exhibit a Wnt-ON/Notch-OFF response but how is this response generated? Now, on p. 4405, Keith Brennan and co-workers report that Wnt acts via Dishevelled, a key mediator of Wnt/β-catenin signalling, to inhibit the Notch pathway and that this crosstalk controls cell-fate specification during Xenopus epidermal development in vivo. Dishevelled, they report, binds and directly inhibits the CSL (CBF1, Suppressor of Hairless, Lag-1; RBPJκ in mice) transcription factors that mediate Notch signalling. Moreover, this crosstalk mechanism is conserved between vertebrates and invertebrates. Thus, by acting as both an activator of Wnt signalling and an inhibitor of Notch signalling, Dishevelled sharpens the distinction between opposing Wnt and Notch responses, thereby ensuring that robust cell-fate decisions are taken during development.

MicroRNA regulation of angiogenesis

Secreted signalling molecules regulate cellular communication across tissues during development. For example, during angiogenesis in zebrafish embryos, vascular endothelial growth factor (VEGF) secreted by muscle controls the expansion and remodelling of the vascular network. The precise regulation of VEGF expression in muscle cells is therefore essential for angiogenesis but how is this regulation achieved? On p 4356, Yuichiro Mishima, Antonio Giraldez and colleagues report that miR-1 and miR-206 (miR-1/206; two conserved microRNAs with similar sequences) negatively regulate angiogenesis during zebrafish development. The negative effect of miR-1/206 on angiogenesis, the researchers report, is mediated in part by direct regulation of VegfAa in muscle. Thus, masking the target sites for miR-1/206 in the 3′ UTR of vegfaa has a pro-angiogenic effect similar to that of miR-1/206 knockdown. By contrast, reducing the levels of VegfAa rescues the increase in angiogenesis produced by miR-1/206 knockdown. Together, these findings uncover a novel function for miR-1/206 in the control of developmental angiogenesis and highlight a key role for microRNAs as regulators of cross-tissue signalling.

HIF1α controls collagen secretion under hypoxia

The production of collagen – a major component of the extracellular matrix – depends on hydroxylation of proline residues, a reaction that uses molecular oxygen as substrate. Cells that develop in hypoxic settings can nevertheless produce collagen during embryogenesis. Elazar Zelzer and colleagues begin to resolve this paradox on p. 4473 by identifying the transcription factor hypoxia-inducible factor 1 α (HIF1α) as a central regulator of collagen hydroxylation and secretion by chondrocytes in the hypoxic growth plate of developing mouse bones. The researchers show that Hif1a loss of function in growth plate chondrocytes arrests the secretion of collagen. Hif1α, they report, drives the transcription of collagen prolyl 4-hydroxylase, which mediates collagen hydroxylation and its subsequent folding and secretion. Concurrently, Hif1α also maintains cellular oxygen levels, probably by controlling the expression of pyruvate dehydrogenase kinase 1, an inhibitor of the tricarboxylic acid cycle. This two-pronged mechanism, the researchers suggest, allows chondrocytes to secrete large amounts of collagen in the hypoxic environment of the growth plate.

Cycling towards sepal cell specification

During development, cell division and cell specification must be coordinated to ensure that the tissues and organs of the adult organism are the correct size and contain the right proportions of various cell types. Here (p. 4416), Adrienne Roeder and colleagues investigate how developmental regulators interact with the cell cycle in Arabidopsis sepals to create a characteristic pattern of outer epidermal cells in which elongated giant cells, which are produced by endoreduplication (DNA replication without cell division), are interspersed between small cells, which divide mitotically. They show that distinct enhancers are expressed in giant cells and small cells, which suggests that these cells have different identities as well as different sizes and ploidies. Several members of the epidermal specification pathway control the identity of giant cells, they report, which is established upstream of cell-cycle regulation. By contrast, endoreduplication represses small cell identity. Thus, suggest the researchers, cell type affects cell-cycle regulation but, in addition, cell-cycle regulation can control cell identity.

Epigenetic reprogramming egged on

The generation of cloned embryos by somatic cell nuclear transfer (SCNT) into enucleated mature oocytes is inefficient because epigenetic reprogramming is limited in these embryos. However, treatment of somatic nuclei with a cytoplasmic extract from germinal vesicle (GV) stage oocytes before SCNT is known to improve the efficiency of cloned mouse production. Now (p. 4330), Hong-Thuy Bui, Jin-Hoi Kim and co-workers use an extract from GV stage pig oocytes (GVcyto-extract) to investigate epigenetic reprogramming events in pig fibroblasts. The researchers report that fibroblasts treated with GVcyto-extract express the stem cell-associated proteins Oct4 and Nanog and re-differentiate into three primary germ cell layers in vitro and in vivo. Moreover, the use of donor nuclei treated with GVcyto-extract, they report, increases the number of high quality SCNT-generated blastocysts that exhibit levels of histone H3-K9 methylation and acetylation and Oct4 and Nanog expression similar to those in embryos fertilised in vitro. These results suggest that a combination of epigenetic reprogramming techniques might improve the efficiency of development in SCNT-generated embryos.

Notch pick and mix regulates lung development

In developing mammalian lungs, the terminal buds of elongating airways contain multipotent epithelial progenitors that give rise to the three major lung epithelial cell types: Clara, ciliated and neuroendocrine (NE) cells. Now, Mitsuru Morimoto and colleagues provide new insights into how Notch signalling regulates the distribution of these epithelial cell types in the airway (see p. 4365). Using stepwise removal of Notch1, Notch2 and Notch3 from developing mouse lung epithelium, the researchers show that Notch2 alone mediates the Clara/ciliated cell fate decision whereas all three receptors regulate NE fate selection in an additive manner. All three Notch receptors also additively regulate the size of the presumptive pulmonary neuroepithelial body (pNEB; NEBs are clusters of NE cells) through mutual interactions between NE cells and a population of SSEA-1 (stage-specific antigen 1)-positive epithelial cells that surrounds the pNEB. These and other results indicate that two different assemblies of Notch receptors control the number and distribution of lung epithelial cell types during lung organogenesis.

PLUS…

Drosophila neuroblasts: a model for stem cell biology

As part of the ‘Development: the big picture’ series, Homem and Knoblich explain why Drosophila neuroblasts, the stem cells of the developing fly brain, have emerged as a key model system for neural stem cell biology. See the Primer on p. 4297

Endocytic receptor-mediated control of morphogen signaling

Thomas Willnow and colleaugues describe how low-density lipoprotein receptor-related proteins (LRPs) constitute central pathways that modulate morphogen presentation to target tissues and cellular signal reception, and how LRP dysfunction leads to developmental disturbances in many species. See the Primer on p. 4311

Piecing together the vertebrate skull

As part of the ‘Development Classics’ series, Nicole M. Le Douarin discusses her 1993 Development paper, in which she and her colleagues used the quail-chick chimera system to decipher the embryonic origin of the bones of the head skeleton of the avian embryo. See the Spotlight article on p. 4293

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Last week, the Royal Society hosted a meeting entitled “Regulation from a distance: Long-range control of gene expression in development and disease”. The impressive London offices of the Society (complete with double helix-inspired door handles) added a sense of occasion to what was bound to be a fascinating meeting, based on the list of excellent speakers from Europe and the US. It brought together a diverse range of scientists from different backgrounds, united by their common interest in the vast stretches of non-coding DNA in the genome, and the role that it plays in gene regulation. I’ll try to give a general overview of the talks here, but with greater focus on the developmental biology topics.

The meeting was opened by Robert Hill (University of Edinburgh) who described an extreme example of a long-range enhancer: the ZRS enhancer that drives Shh expression in the developing limb bud from a distance of 1 Mb. His group have studied this enhancer’s role in preaxial polydactyly (extra digits). The talks that followed showcased a range of useful methods for identifying such distant enhancers.

Francois Spitz (EMBL) described the use of the GROMIT method (Genome Regulatory Organisation Mapping with Integrated Transposons) to mobilise a transposon containing a “regulatory sensor” around the genome, giving reporter gene expression as a read-out of the tissue-specific enhancer signals at different genomic locations. Ivan Ovcharenko (NIH) presented a computational approach for identifying such tissue-specific enhancers, showing that the lessons learned from analysing the tissue-specific transcription factors bound at promoters are also useful for locating enhancers of those tissue-specific genes. Joanna Wysocka (Stanford) presented a combination of different approaches for enhancer identification including conservation analysis, identification of DNaseI hypersensitive sites and ChIP-seq for histone modifications and p300. Her group has used these methods to identify neural crest-specific enhancers that drive novel regulators of craniofacial development.

Several speakers discussed the chromosome conformation capture or ‘C’ techniques, which are used to visualise physical interactions between distant regions of the genome, such as those between gene promoters and enhancers. Denis Duboule (University of Geneva/EPFL) described the use of 4C to investigate the regulation of the Hoxd gene cluster during limb development, and Wouter de Laat (Hubrecht Institute) spoke about recent advances that have increased the resolution of 4C to allow identification of interactions as close as 10 kb. His group have used this technology to identify a novel enhancer of Oct4 in embryonic stem cells. Wendy Bickmore (University of Edinburgh) emphasised the importance of corroborating findings from the ‘C’ techniques using other methods, such as FISH (fluorescent in situ hybridisation).

Although many methods can be used to identify potential enhancers, it is also important to validate their activity. Len Pennacchio (LBNL) described recent efforts in compiling the VISTA enhancer browser: a publicly available database of enhancers that drive tissue-specific gene expression in vivo in transgenic mouse embryos.

In addition to the developmental biology talks, several speakers focused on the role of long-range gene regulation in human disease, which is an important consideration given that many mutations associated with disease are in non-coding regions of the genome. We heard from Doug Higgs (University of Oxford) on the alpha-globin genes and thallasaemias, Matthew Freedman (Harvard) on the Cancer Genome Atlas, and Gioacchino Natoli (European Institute of Oncology) on enhancers that are activated during the inflammatory response.

Enhancer mutations are also an important driving force in evolution, as was explained by David Kingsley (HHMI/Stanford) who studies the rapid natural evolution of three-spined stickleback populations as they colonise new lakes. Major phenotypic changes in these populations can be explained by mutations in a few key developmental genes, but while mutations that inactivate these genes are lethal, mutations in enhancers can alter their expression patterns in a way that produces an evolutionary advantage. Also on the population genetics theme, Manolis Dermitzakis (University of Geneva) presented human data that he has used to investigate the genetic and epigenetic contributions to gene expression.

The majority of speakers focused on long-range gene regulation by enhancers, but it is also important to consider the role of insulators. This was addressed by Bing Ren (UCSD) in the final talk of the meeting. His group have used the HiC technique to create a genome-wide map of chromosomal interactions, revealing that the three-dimensional structure of the genome is divided in to topological domains. Many long-distance interactions occur within these domains, but very few occur between different domains, suggesting that the domain boundaries represent insulators. However, the role of CTCF as an insulator factor was debated at length in the subsequent discussion session.

The Royal Society meetings have an unusual format, with sets of two half-hour talks followed by half an hour of discussion. These long discussion sessions allowed for much deeper probing of the subject, and encouraged debate that became quite lively at times, showing just how exciting this area of research is! Some topics were revisited throughout the meeting, establishing links between different areas, which is important in driving the field forward. The variety of experimental approaches presented showed how insights can be gained by investigating regulation of gene expression at multiple levels. In his talk, John Stamatoyannopoulos (University of Washington) mentioned that the genome contains over 4 million distinct elements that are associated with gene regulation, so there is clearly a lot of work to be done in this field in the future!

The Royal Society have made the audio of all talks from this meeting available online, so check their website for more details on any of the presentations.

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The big news this past month was of course the announcement of the Nobel Prize for Physiology and Medicine, awarded to John Gurdon and Shinya Yamanaka for their work on cellular reprogramming. Katherine Brown wrote a brief post about John Gurdon’s connection to the Company of Biologists – and to the Node!

Hydra Summer School
A few weeks before the Nobel committee acknowledged the importance of the field of stem cells, the Hydra Summer School in Stem Cells and Regenerative Medicine trained a group of young researchers in the field. Two participants wrote about their experience and the highlights of the course. Sarah-Jane writes:

“Personally, I particularly enjoyed the recurring discussion on the definition of a stem cell, and how one must be aware of the language subtleties that exist between different researchers and sub-disciplines. Is a fertilised egg a stem cell? Perhaps, if you’re a developmental biologist.”

Kif added: “There is a huge prospect for the field of stem cell biology in the future, from understanding to alleviating diseases. Lectures and discussions at the summer school gave me confidence that this is a realistic goal.”

Development: Past, Present and Future
Stem cells were also one of the topics discussed at Development’s 25th anniversary symposium. That’s not surprising, considering the theme was “Past, Present and Future”! Several of Development’s current and former editors took the stage to talk about their work, and the day ended with a panel discussion.

Nucleitracker
But let’s not forget the many other advances in the field of developmental biology, that help us study multicellular tissues and organisms. Improvements in imaging and automation techniques are another important development, and we also covered that on the Node this past month: Andrew Chisholm’s group created a method to track nuclei in the developing C. elegans embryo, including the later stages.

“Claudiu Giurumescu, a postdoc in my lab, took a different approach to the problem of tracking nuclei in the crowded environment of the later embryo. Importantly, he decided to use a combination of automatic tracking and manual curation. The tracking relies on the predictable behavior of nuclei in worm embryos: most nuclei do not move around much on the time scale used in 4D movies. Claudiu devised algorithms that took advantage of this predictability to search locally for each nucleus at a given time point, based on the information on where the nucleus is at the previous time point.”

Also on the Node:

–Interview with Jiri Friml
–Interview with Linda Partridge
–Worms teach about germline stem cells
–Publishing in the biomedical sciences: if it’s broken, fix it!

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Last month was an exciting one for stem cell research. I’m sure you all saw how stem cells hit international headlines with the announcement of a Nobel Prize for John B. Gurdon and Shinya Yamanaka. I thought you might be interested to read reactions from several leading scientists and check out a film clip about the prize-winning work in our blog on eurostemcell.org

We’ve got a new short film all about reprogramming and iPS cells coming very soon too. Watch out for it going live on our website www.eurostemcell.org at the end of November or early December, in time for the formal Nobel Prize awards ceremony of 10th December.

We were also celebrating in October because it was Stem Cell Awareness day on the 3rd of the month. Researcher Christèle Gonneau helped us celebrate the day by giving us a great window into life as a stem cell biologist on twitter. You can check out a summary of her day’s tweets and pictures in our blog too.

You can keep up to speed on stem cell news and our activities by following @eurostemcell on Twitter or, if Twitter’s not your thing, say hello to us on Facebook or our website.

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Last Thursday, Development organized a one-day symposium, “Past, Present and Future”, to mark the 25th anniversary of the journal. All speakers were past or present editors of Development, and the work they presented gave a broad overview of the current status of the field.

At the end of the day, a panel discussion looked back at the advancements in developmental biology of the past 25 years, but mostly considered the future of the field. A collection of tweets summarizing the panel discussion is at the end of this post, below the photos. (And if you’re viewing this on the front page of the Node, make sure to click through to the second part of the post to see the remaining photos!)

Kenneth Chien spoke about driving heart progenitor cell fate and regeneration in vivo via chemically modified mRNA – a technique that could have direct therapeutic applications.

Peter Lawrence gave a historical overview of planar cell polarity signalling, as well as discussing his latest insights into how cells orient in a tissue (published recently in Development!).

Magdalena Götz highlighted radial glial cells’ function as stem and progenitor cells in the brain, providing insights into how particular patterns of self-renewal vs. differentiation might define brain region size.

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At the EMBO meeting in late September, Linda Partridge gave a keynote talk about the role of the nutrient-sensing insulin/IGF/TOR signaling network in ageing. In her talk she showed, for example, how dietary restriction extends the lifespan of several organisms. I had a chance to talk to her later that day, and I asked her some questions about her research.

What made you decide to study the biology of ageing?

I started out as an evolutionary biologist, and from an evolutionary point of view, ageing is really weird, because it’s a trait that lowers fitness. An animal that didn’t become more likely to die or less fertile with age would leave more offspring. Yet we know that ageing evolves to happen at very different rates in the natural world. If you just look at mammals, some whales can live 200 years, but a shrew can only make a few weeks. It’s a real paradox, and that’s how I got interested in it.

Initially I did a lot of work with Drosophila, looking at the two possible routes to the evolution of ageing: the accumulation of deleterious mutations that only affect the late part of life, and processes that are advantageous to the young but then overshoot in the old and cause problems. In the fly we’ve got quite a lot of evidence for the latter: there’s a distinct trade-off between events early and late in life.  That piqued my interest in mechanisms of ageing.

What, if anything, is the overlap of processes that are involved in both development and ageing?

Well, I often say that ageing is not at all like development, because development is a beautifully programmed process: It’s a well-oiled machinery with a hierarchy of genes that make sure that the right things happen in the right place at the right time and that they happen in the same way in all developing system. Ageing is much more haphazard than that, because it’s essentially a side-effect in evolution. It’s a result of the inability of natural selection to hold things together as the animal gets older. So it presents a much more complex picture of different things going on in different individuals and at different times. We don’t think it’s adaptive at all: there aren’t mechanisms that have evolved to cause damage and death.

However, I think some of the things that happen during development – particularly mechanisms promoting growth and cell division – can be the very processes that remain too active and cause problems as the animal gets older. That’s called the hyperfunction idea of ageing, and a very obvious case is cancer.

During your talk you mentioned that it’s really hard to do dietary restriction research in humans because they won’t stick to the diet. Are there any post-hoc population studies?

There have been quite a few comparisons of simply different cultures of humans. Of course that’s always going to just be a correlation, because there could be other differences. But the most remarkable example is the Japanese – in particular the Okinawan Japanese. Okinawa is an island group in the south of the Japanese archipelago, where, seemingly just as a cultural habit, people eat much less than the mainland Japanese, who already eat considerably less than the rest of the world. It just so happens that the Okinawans have the highest proportion of people reaching the age of 100 in the world, and the Japanese mainland is next in line. I think it’s just a correlation, but it’s interesting that their intake is so low and their lifespans are so long – perhaps there is some causal connection between them.

If you had unlimited time and unlimited money, what would you like to research?

I would be very interested in getting directly involved in research with humans that keeps them healthier for longer. That’s the aim of this line of research: to find ways of keeping people healthy in the later part of life so that the period of ill health at the end of life is at least shortened and preferably abolished. One thing I’d like to do are clinical trials, particularly with one or two drugs that already exist for particular conditions but that I suspect – and in fact it’s starting to be demonstrated – have a much wider therapeutic range than has been previously supposed. These two drugs are aspirin and metformin, which is used as a first line of defence against type II diabetes.

So I would be interested in working with humans, but I think there also has to be quite a lot of experimental animal work underpinning that. I would very much like to make new mutant mice for various components of the nutrient sensing pathway, and see how mouse models of slowed ageing interact with specific models of human ageing-related diseases. That’s obviously terribly expensive, so at the moment the sky is definitely not the limit. And in invertebrates, particularly the fly, which is my great favourite, I’d like to do some fast work on basic mechanisms. We don’t really understand what ageing is, and I would love to be able to proceed faster with elucidating the mechanisms of ageing.

All of these things are always resource-limited. That’s what sets the limit on the rate of progress.

Do you have any general advice for students and postdocs who are just starting their research career?

Pick something that you’re interested to work on, because no matter how interested you are and how passionate you are about science, you’re going to have bad moments. There’s a large element of luck in science: Sometimes you do experiments that don’t work. It can be frustrating, it can be slow, so you’d better be interested in the first place. Pick a lab that’s really going to look after you and give you good projects and support you in your projects. Think about the development of your career – whatever that’s going to be. It might be into science, or it might be into something where scientific training is really useful. Whatever it is, you want a mentor who is thinking about you and your future interests.

For female scientists there can sometimes be additional complications. Two-job problems in partnerships apply to everyone, not just women, but sometimes I think women feel them more. The decision when to have kids, can also be a difficult one. Most of the female students and postdocs who pass through my lab do have children while they’re there. I suppose my observation on that is that it’s actually easier the younger you do it. It’s much easier to interrupt a PhD than to be confronted with starting a family and starting your own lab for the first time. I think that’s quite a tough thing to do.

To see Linda Partridge’s answer to my final question, about preparing for conference presentations, see this previous post with speaking tips.

To read more about her keynote lecture, see this article in The Telegraph.

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On my desk sits a tattered photocopy of one of the pinnacles of modern developmental biology, the “embryonic lineage” paper by John Sulston, et al. (1983). In this paper, Sulston et al. completed a project begun in the late 19th century, namely to trace the complete genealogy of all cells in a nematode embryo. C. elegans, like many (although not all) nematodes, displays highly invariant development, each embryo developing to an adult with the same number of cells. The introduction of Nomarski DIC optics in the 1960s made it possible for the first time to trace all cells and their divisions in live embryos, a feat performed without significant aid from video recording, and well before the era of computer image analysis.

The ‘embryonic lineage’ paper remains a treasure trove of information and insights. Yet many aspects of development are not captured in the lineage, most significantly information on dynamic movements and neighbor relationships. Further, the extreme amount of time and patience required in manual cell lineage analysis meant that much of this knowledge was unused for several years until video recording allowed manual tracking of cells from a single embryo with tools such as Simi Biocell. A breakthrough was the development by Zhirong Bao and Bob Waterston’s lab of computer based tracking algorithms to automatically trace cell lineages from 4D movies of nuclear histone-GFP markers.

Completely automated nuclear tracking is highly efficient in the early embryo (up to 350 cells). The processes of most interest to my lab, including ectodermal morphogenesis and organogenesis, begin after 350 cells, and our efforts to automatically track cells in these later stages were unsuccessful. Essentially the embryo becomes too crowded with nuclei; also, compromises in illumination and image quality needed to avoid phototoxicity in long 4D confocal movies result in images that are not clear enough for complete automation to be efficient.

Claudiu Giurumescu, a postdoc in my lab, took a different approach to the problem of tracking nuclei in the crowded environment of the later embryo. Importantly, he decided to use a combination of automatic tracking and manual curation. The tracking relies on the predictable behavior of nuclei in worm embryos: most nuclei do not move around much on the time scale used in 4D movies. Claudiu devised algorithms that took advantage of this predictability to search locally for each nucleus at a given time point, based on the information on where the nucleus is at the previous time point. Of course, this means the user has to identify all nuclei at the first time point in the series, which is usually easy for an early embryo. As all nuclei are either tracked or flagged for curation at each time point, error propagation is minimized.

We first tried this approach on embryos imaged with conventional laser scanning confocal microscopy, and were able to successfully follow all nuclei up to the point at which embryonic muscle movements interfere with tracking, a time when all but four nuclei have been generated in the embryo. Sukryool (Alan) Kang, a student with Pam Cosman, played a major role in refining the visualization tools and in quantitative analysis of cell movements. The resulting dynamic models of the embryo can be visualized in a variety of ways, as shown (at rather low resolution) in our Supplementary Material, and at higher resolution on our lab web page: http://132.239.70.11/~wormlab/. Our Matlab code and user manuals are publicly available on Sourceforge. We are still refining the visualization tools and plan to integrate 4D movies more directly with the lineage tree.

We next wanted to assess the generality of our semiautomated approach. We first collaborated with Thomas Planchon and Eric Betzig (Janelia Farm), whom we had met one summer at the MBL in Woods Hole while they were demonstrating their novel structured illumination approach, Bessel beam microscopy. Bessel beam illumination has much higher z-resolution than standard confocal movies, with reduced phototoxicity. Fortunately, Bessel beam 4D movies of C. elegans embryos proved highly amenable to our semiautomated tracking.

How well does this approach work in samples where development is less predictable?  To answer this we struck up a collaboration with Debbie Yelon’s lab, our neighbors at UCSD, who were interested in tracking nuclei in zebrafish cardiac morphogenesis. Using data generated by Josh Bloomekatz, our tracking algorithms were able to track large numbers of zebrafish nuclei with only minor modifications.

Our work adds another tool to the toolbox for anyone interested in tracking large sets of nuclei, or similar features, in complex samples. Fully automated tracking remains the method of choice in simple samples where nuclei are well separated and can be unambiguously tracked from frame to frame. Semiautomated tracking allows one to go further into development, and opens up the prospect of quantitative analysis of morphogenetic stages of embryogenesis.

Sulston JE, Schierenberg E, White JG, Thomson JN. The embryonic cell lineage of the nematode Caenorhabditis elegans. Dev Biol. 1983 Nov;100(1):64-119. PubMed PMID: 6684600. (Full text at WormAtlas)

Bao Z, Murray JI, Boyle T, Ooi SL, Sandel MJ, Waterston RH. Automated cell lineage tracing in Caenorhabditis elegans. Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2707-12. Epub 2006 Feb 13. PubMed PMID: 16477039; PubMed Central PMCID: PMC1413828.

Giurumescu, C.A., Kang, S., Planchon, T.A., Betzig, E., Bloomekatz, J., Yelon, D., Cosman, P. & Chisholm, A.D. (2012). Quantitative semi-automated analysis of morphogenesis with single-cell resolution in complex embryos, Development, 139 (22) 4279. DOI: 10.1242/dev.086256

(2 votes)

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Post-doc position in Developmental Biology

Development and evolution of median fin in chordates

     A post-doc position is available in the group of Sébastien DARRAS. The group has been recently established at the marine station of Banyuls-sur-mer (Mediterranean coast, close to the Spanish border). We are interested in the molecular control of the ascidian Ciona intestinalis embryogenesis. Our focus is on gene networks involved in patterning and differentiation of larval tail epidermis that gives rise to peripheral sensory neurons and median fin blades. We want to use the diversity of basal marine chordate species (tunicates and cephalochordates) available at the marine station to probe the evolution of median fin formation.

The post-doctoral research project aims at comparing at genomic and functional levels the  same developmental process in multiple species. Strong background in developmental and molecular biology, as well as curiosity are required. Experience with non-conventional model organisms will be appreciated.

Funding by the French Research Agency (ANR) is avalaible immediately, but applicants are expected to apply for their own financial support.

Contact: Sébastien DARRAS (sebastien.darras@obs-banyuls.fr)

Laboratoire de Biologie Intégrative des Organismes Marins (BIOM)
UMR7232 CNRS-INSB-UPMC
Observatoire Océanologique de Banyuls
Avenue du Fontaulé
66650 Banyuls-sur-mer
FRANCE

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Postdoc position in appendage skeletal development and evolution

An NSF funded postdoctoral position of up to 3 years, is available from 1 February 2013 to work with Dr Marcus C. Davis on the evolution of Hox gene regulation in fin and limb skeletal patterning, using North American paddlefish (Polyodon spathula) and axolotl (Ambystoma mexicanum) as model systems.

Applicants must have a PhD in an area of biology with strengths in developmental biology.  The qualified applicant will have significant experience in molecular biology techniques including: PCR techniques, primer design, antisense riboprobe construction, whole mount in situ hybridization. Experience working with aquatic vertebrate embryos and larvae (zebrafish, axolotl, Xenopus, and/or non-model taxa) is essential, as are basic histology skills. Experience with focal electroporation and microinjection tecnhniques in aquatic vertebrates is highly desired for this project.  Additionally, experience with transcriptome data and analysis will be an advantage.  Applicants must be prepared for extensive and rigorous experimental hours during the short embryonic growth season (April and May) for paddlefish.

Send applications including a CV, a statement of research interests and the names of three references to: Dr Marcus Davis, Dept. of Biology and Physics, 1000 Chastain Road, Bldg #12, Kennesaw, GA 30144.  Electronic submissions are preferred and can be sent to: mdavi144@kennesaw.edu.

Kennesaw State Official Job Posting: https://kennesaw.hiretouch.com/job-details?jobID=9624&job=postdoctoral-fellow

Davis Lab Page: http://science.kennesaw.edu/~mdavi144/Main_Page.html

Kennesaw State University is an Affirmative Action/Equal Opportunity Employer and Educator. Georgia is an Open Records State.

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