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10 thoughts on “A Day in the Life of…a Xenopus lab”

    1. We use 3% agarose and put the backside of a ping pong paddle coating. You have to try the right size of the knob

    1. Hi,

      They are from two different females – the pigmentation varies from female to female. It doesn’t generally mean much, although general consensus is that darker, and especially even and not patchy/mottled pigmentation, tend to fertilize better.

  1. Hi,
    I’m a PhD student whose focus is more on ecology than biochemistry. But I have a quick question for you since I have recently started using Xenopus laevis embryos. You may not be able to answer this, but it’s worth a shot.
    During early stage embryonic development, do X. laevis embryos exhibit similar behavior to that of other frog species (e.g. Rana temporaria)? Does the jelly capsule absorb water from the surroundings causing the jelly to swell, or is there very little change in the size of the embryos as development continues?

    1. Hi,

      This is a good question, and maybe a Rana person would be better equipped to answer it/compare. The embryo does increase dramatically in size before ingesting any food, and this is largely due to water uptake. I don’t believe this is due to the jelly itself, but in the lab we always remove the jelly coat reasonably early on in embryo development so I can’t be sure.

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