Seema Grewal, Author at the Node

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 142, Issue 8)

Posted by Seema Grewal, on 7 April 2015

Here are the highlights from the current issue of Development: Getting to the heart of human epicardial differentiation The epicardium is crucial for heart development and function, and it ...

In Development this week (Vol. 142, Issue 7)

Posted by Seema Grewal, on 24 March 2015

Here are the highlights from the current issue of Development: ActivinA-ting spiny neuron production from hPSCs The medium-sized spiny neurons, the main projection neurons of the striatum, are generated ...

In Development this week (Vol. 142, Issue 6)

Posted by Seema Grewal, on 10 March 2015

Here are the highlights from the current issue of Development: Dmrt1: a common thread in sex determination Dmrt1 and its related genes play a key role in sex determination ...

In Development this week (Vol. 142, Issue 5)

Posted by Seema Grewal, on 24 February 2015

Here are the highlights from the current issue of Development: pancRNAs in early development Promoter-associated noncoding RNAs (pancRNAs) are a class of long noncoding RNAs, transcribed from bidirectional promoters ...

In Development this week (Vol. 142, Issue 4)

Posted by Seema Grewal, on 10 February 2015

Here are the highlights from the current issue of Development: Pathways to human hypothalamic neurons The dysfunction of hypothalamic neurons is implicated in a number of common diseases, including ...

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps

by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010