Seema Grewal, Author at the Node

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 142, Issue 13)

Posted by Seema Grewal, on 30 June 2015

Here are the highlights from the current issue of Development: Controlling Traffic jam for niche specification Tissue stem cells are dependent on their local microenvironment – the niche – ...

In Development this week (Vol. 142, Issue 12)

Posted by Seema Grewal, on 16 June 2015

Here are the highlights from the current issue of Development: New tool for myelin formation in vitro The myelination of axons by oligodendrocytes in the nervous system is crucial for ...

In Development this week (Vol. 142, Issue 11)

Posted by Seema Grewal, on 26 May 2015

Here are the highlights from the current issue of Development: Hippo signalling: not just for growth The Hippo signalling pathway regulates organ growth: activation of the pathway inhibits proliferation ...

In Development this week (Vol. 142, Issue 10)

Posted by Seema Grewal, on 12 May 2015

Here are the highlights from the current issue of Development: Capping off sesamoid bone development Sesamoid bones are small, flat bones that are embedded within tendons. To date, it ...

In Development this week (Vol. 142, Issue 9)

Posted by Seema Grewal, on 28 April 2015

Here are the highlights from the current issue of Development: The light at the end of the tubule Tubular structures, such as kidney tubules or blood vessels, carry out ...

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps

by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010