Seema Grewal, Author at the Node

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 142, Issue 3)

Posted by Seema Grewal, on 20 January 2015

Here are the highlights from the new issue of Development: Invadosomes: aiding axonal invasion Invasive cells such as immune and metastatic cancer cells form protrusions known as invadosomes, which ...

In Development this week (Vol. 142, Issue 2)

Posted by Seema Grewal, on 6 January 2015

Here are the highlights from the new issue of Development: The ‘second brain’: taking gut development up a Notch The vertebrate gastro-intestinal (GI) tract consists of a regionalized epithelial ...

In Development this week (Vol. 142, Issue 1)

Posted by Seema Grewal, on 16 December 2014

Here are the highlights from the current issue of Development: Planar cell polarity squeezes in on the action The planar cell polarity (PCP) pathway regulates the polarization of epithelial ...

In Development this week (Vol. 141, Issue 24)

Posted by Seema Grewal, on 2 December 2014

Here are the highlights from the current issue of Development: Assessing the evolutionary origin of neural progenitors The nervous system of bilaterians arises from a small pool of neural ...

In Development this week (Vol. 141, Issue 23)

Posted by Seema Grewal, on 18 November 2014

Here are the highlights from the new issue of Development: Akt acts to reprogram germ cells Primordial germ cells (PGCs) are unipotent – they go on to form germline ...

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps

by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010