Seema Grewal, Author at the Node

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 142, Issue 18)

Posted by Seema Grewal, on 22 September 2015

Here are the highlights from the current issue of Development – our Special Issue on “Human Development”. This Special Issue brings together a collection of Reviews and Research Articles that directly ...

In Development this week (Vol. 142, Issue 17)

Posted by Seema Grewal, on 1 September 2015

Here are the highlights from the current issue of Development: Tubulogenesis at single-cell resolution Formation of the blood vessels involves complex endothelial morphogenesis, including directional cell migration and lumen ...

In Development this week (Vol. 142, Issue 16)

Posted by Seema Grewal, on 18 August 2015

Here are the highlights from the current issue of Development: Probing gene expression dynamics in stem cells The accurate control of gene expression is essential for cell differentiation during ...

In Development this week (Vol. 142, Issue 15)

Posted by Seema Grewal, on 4 August 2015

Here are the highlights from the current issue of Development: CHD4 restrains gene expression during lineage specification The first lineage-specification event in the early embryo generates the inner cell ...

In Development this week (Vol. 142, Issue 14)

Posted by Seema Grewal, on 21 July 2015

Here are the highlights from the current issue of Development: HIF1α muscles in on regeneration During early development, skeletal muscle stem/progenitor cells (SMSPCs) are thought to reside in low ...

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps

by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010