Seema Grewal, Author at the Node

Seema Grewal

Posts by Seema Grewal

In Development this week (Vol. 142, Issue 23)

Posted by Seema Grewal, on 1 December 2015

Here are the highlights from the current issue of Development: Human embryos: a mixed bag Human embryonic stem cells (hESCs) are conventionally derived from embryos that are at the ...

In Development this week (Vol. 142, Issue 22)

Posted by Seema Grewal, on 17 November 2015

Here are the highlights from the current issue of Development: Nodal: sustaining Shh expression Underlying the developing vertebrate forebrain is the prechordal mesoderm, which secretes sonic hedgehog (Shh) at ...

In Development this week (Vol. 142, Issue 21)

Posted by Seema Grewal, on 3 November 2015

Here are the highlights from the current issue of Development: Spatial mapping in the brain Many sensory systems show topographic mapping: the spatial organisation of peripheral receptors is preserved ...

In Development this week (Vol. 142, Issue 20)

Posted by Seema Grewal, on 20 October 2015

Here are the highlights from the current issue of Development: Atoh1: earmarked for differentiation Atoh1 is a key regulator of the differentiation of hair cells, the sensory cells that ...

In Development this week (Vol. 142, Issue 19)

Posted by Seema Grewal, on 6 October 2015

Here are the highlights from the new issue of Development: Hormone-mediated flower development: a HEC of a job Fruits originate from the female reproductive part of the flower, the ...

Hi Gary. We used to fix mouse embryos (both pre- and post-implantation) in PFA then methanol. After your standard PFA fix, wash in PBS then pass them through 25%, 50%, 75% then 100% methanol (in PBS, 5mins each step). To rehydrate, just do the reverse: 75%, 50%, 25% then back into PBS. This worked fine for me and others in the lab (the embryos do get a little sticky while in the methanol, though!). Hope this helps

by Seema Grewal in Protocol: Rehydrating embryos once fixed and dehydrated… on July 9, 2010